In situ intracellular calcium oscillations in osteocytes in intact mouse long bones under dynamic mechanical loading

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Da JingX Edward Guo

Abstract

Osteocytes have been hypothesized to be the major mechanosensors in bone. How in situ osteocytes respond to mechanical stimuli is still unclear because of technical difficulties. In vitro studies have shown that osteocytes exhibited unique calcium (Ca(2+)) oscillations to fluid shear. However, whether this mechanotransduction phenomenon holds for in situ osteocytes embedded within a mineralized bone matrix under dynamic loading remains unknown. Using a novel synchronized loading/imaging technique, we successfully visualized in real time and quantified Ca(2+) responses in osteocytes and bone surface cells in situ under controlled dynamic loading on intact mouse tibia. The resultant fluid-induced shear stress on the osteocyte in the lacunocanalicular system (LCS) was also quantified. Osteocytes, but not surface cells, displayed repetitive Ca(2+) spikes in response to dynamic loading, with spike frequency and magnitude dependent on load magnitude, tissue strain, and shear stress in the LCS. The Ca(2+) oscillations were significantly reduced by endoplasmic reticulum (ER) depletion and P2 purinergic receptor (P2R)/phospholipase C (PLC) inhibition. This study provides direct evidence that osteocytes respond to in situ mechanical load...Continue Reading

References

Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Klein-NulendE H Burger
May 1, 1997·The American Journal of Physiology·C T HungI M Shapiro
Jan 8, 1999·Journal of Biomechanics·C R JacobsH J Donahue
Jan 14, 1999·The American Journal of Physiology·N E AjubiP J Nijweide
May 4, 2000·American Journal of Physiology. Cell Physiology·N X ChenR L Duncan
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N R JørgensenT H Steinberg
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Aug 1, 2002·Calcified Tissue International·C H TurnerD B Burr
Jul 16, 2003·European Journal of Pharmacology·Junko YoshidaMatomo Nishio
Nov 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuefeng HanSheldon Weinbaum
Jun 30, 2005·Journal of Bone and Mineral Metabolism·Charles H Turner, Alexander G Robling
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Liyun WangMitchell B Schaffler
Nov 5, 2005·The Journal of Biological Chemistry·Jiliang LiCharles H Turner
Feb 16, 2007·Journal of Cellular Physiology·Damian C GenetosHenry J Donahue
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·B Ian Hutchins, Katherine Kalil
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Feb 19, 2008·Bone·Lynda F Bonewald, Mark L Johnson
May 8, 2009·Orthodontics & Craniofacial Research·S JudexC Rubin
Aug 12, 2009·Journal of Biomechanics·Taiji AdachiHiroshi Kamioka
Oct 13, 2009·Critical Reviews in Eukaryotic Gene Expression·Alexander G Robling, Charles H Turner
Jan 15, 2010·Annual Review of Fluid Mechanics·Susannah P Fritton, Sheldon Weinbaum
Mar 6, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·G Y RochefortC L Benhamou
May 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael D Brodt, Matthew J Silva
Jun 26, 2010·Journal of Applied Physiology·Maureen E LynchMarjolein C H van der Meulen
Jul 14, 2010·Annual Review of Biomedical Engineering·Christopher R JacobsAlesha B Castillo
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sirisha BurraJean X Jiang
Aug 18, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Christopher PriceLiyun Wang
Jan 22, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lynda F Bonewald
Sep 13, 2011·Nature Medicine·Tomoki NakashimaHiroshi Takayanagi
Nov 24, 2011·Molecular Endocrinology·Ning WangAlison Gartland
Nov 25, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·X Lucas LuX Edward Guo

❮ Previous
Next ❯

Citations

Aug 4, 2015·Current Rheumatology Reports·Alesha B Castillo, Philipp Leucht
Jun 2, 2015·Cilia·Kristen L LeeChristopher R Jacobs
Dec 19, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Amy L McNultyFarshid Guilak
Sep 3, 2016·Biomechanics and Modeling in Mechanobiology·Abhishek Kumar Tiwari, Jitendra Prasad
Oct 14, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kevin MiddletonLidan You
Oct 14, 2017·Journal of Bone and Mineral Metabolism·Tomoyo TanakaHiroshi Kamioka
Jan 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yilu ZhouX Lucas Lu
Dec 11, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roman ThalerFranz Varga
Jun 27, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ei Ei Hsu HlaingHiroshi Kamioka
Oct 28, 2017·Aging·LeAnn M Tiede-LewisSarah L Dallas
May 28, 2019·JBMR Plus·Sundar SrinivasanTed S Gross
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jing CaiDa Jing
Mar 19, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Da JingZhuojing Luo
Sep 10, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·David SunMatthew J Silva
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Karl J LewisMitchell B Schaffler
May 28, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·Xueting MeiLidan You
Apr 12, 2019·Scientific Reports·Jitendra Prasad, Ajay Goyal
Oct 28, 2019·Annals of the New York Academy of Sciences·Minyi HuYi-Xian Qin
Mar 27, 2020·Frontiers in Oncology·Zhengfeng YangZhenyao Xu
Jul 25, 2017·Biomechanics and Modeling in Mechanobiology·Ariane ScheurenRalph Müller
Jun 27, 2019·Current Osteoporosis Reports·Chen ZhangNathalie Bravenboer
Oct 18, 2020·International Journal of Molecular Sciences·Anita K Luu, Alicia M Viloria-Petit

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