In situ loading of basic fibroblast growth factor within porous silica nanoparticles for a prolonged release.

Nanoscale Research Letters
Jin ZhangAnualice Thomas

Abstract

Basic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration. However, bFGF is easily degraded in biologic systems. Mesoporous silica nanoparticles (MSNs) with well-tailored porous structure have been used for hosting guest molecules for drug delivery. Here, we report an in situ route to load bFGF in MSNs for a prolonged release. The average diameter (d) of bFGF-loaded MSNs is 57 ± 8 nm produced by a water-in-oil microemulsion method. The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay. The loading efficiency of bFGF in MSNs is estimated at 72.5 ± 3%. In addition, the cytotoxicity test indicates that the MSNs are not toxic, even at a concentration of 50 μg/mL. It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs, e.g. growth factors, to help blood vessel regeneration and potentiate greater angiogenesis.

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Citations

Apr 17, 2012·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Piyush Koria
Oct 8, 2011·Heart Failure Reviews·F R FormigaM J Blanco-Prieto
Oct 12, 2010·Journal of Colloid and Interface Science·Abdul M MuminJin Zhang
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Aug 15, 2019·Nanomedicine·Francisco ArriagadaJavier Morales
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Sep 30, 2018·Advanced Drug Delivery Reviews·Austin P VeithAaron B Baker
May 12, 2021·PloS One·Jesús Alberto Garibay-AlvaradoSimón Yobanny Reyes-López

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Methods Mentioned

BETA
enzyme linked immunosorbent assays
ELISA
enzyme linked immunosorbent assay
Assay
confocal microscopy

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