In Situ Localization of Azospirillum brasilense in the Rhizosphere of Wheat with Fluorescently Labeled, rRNA-Targeted Oligonucleotide Probes and Scanning Confocal Laser Microscopy.

Applied and Environmental Microbiology
B AssmusA Hartmann

Abstract

The colonization of wheat roots by Azospirillum brasilense was used as a model system to evaluate the utility of whole-cell hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes for the in situ monitoring of rhizosphere microbial communities. Root samples of agar- or soil-grown 10- and 30-day-old wheat seedlings inoculated with different strains of A. brasilense were hybridized with a species-specific probe for A. brasilense, a probe hybridizing to alpha subclass proteobacteria, and a probe specific for the domain Bacteria to identify and localize the target bacteria. After hybridization, about 10 to 25% of the rhizosphere bacteria as visualized with 4(prm1),6-diamidino-2-phenylindole (DAPI) gave sufficient fluorescence signals to be detected with rRNA-targeted probes. Scanning confocal laser microscopy was used to overcome disturbing effects arising from autofluorescence of the object or narrow depth of focus in thick specimens. This technique also allowed high-resolution analysis of the spatial distribution of bacteria in the rhizosphere. Occurrence of cells of A. brasilense Sp7 and Wa3 was restricted to the rhizosphere soil, mainly to the root hair zone. C-forms of A. brasilense were demonstrated to ...Continue Reading

References

Feb 1, 1992·Applied and Environmental Microbiology·R I AmannD A Stahl
Oct 1, 1991·Journal of Bacteriology·J R LawrenceD E Caldwell
Mar 1, 1990·Applied and Environmental Microbiology·V TorsvikF L Daae
Nov 1, 1989·Trends in Biochemical Sciences·D Shotton, N White
Jan 1, 1981·Journal of Immunological Methods·G D Johnson, G M Nogueira Araujo
Apr 1, 1994·Journal of Bacteriology·T HurekE Kellenberger
May 1, 1993·Applied and Environmental Microbiology·E L LimE F DeLong
Aug 1, 1993·Applied and Environmental Microbiology·S SpringN Petersen
Feb 1, 1994·Applied and Environmental Microbiology·G M WolfaardtD E Caldwell

❮ Previous
Next ❯

Citations

Jan 14, 2005·Critical Reviews in Microbiology·E SomersM Srinivasan
Aug 30, 2005·Anais Da Academia Brasileira De Ciências·José I Baldani, Vera L D Baldani
Feb 25, 2000·Proceedings. Biological Sciences·G GopalaswamyE C Cocking
Mar 7, 2001·Applied and Environmental Microbiology·S WeberR Conrad
Jan 5, 2000·Applied and Environmental Microbiology·C H Yang, D E Crowley
Aug 3, 2000·FEMS Microbiology Ecology·Thelma CastellanosYoav Bashan
Feb 2, 2000·Applied and Environmental Microbiology·M K Chelius, E W Triplett
May 2, 2000·Applied and Environmental Microbiology·L Pereg GerkI R Kennedy
Jan 20, 2006·FEMS Microbiology Ecology·Patrick K JjembaJodi R Shann
Apr 25, 2006·FEMS Microbiology Ecology·Nancy A FujishigeAnn M Hirsch
May 18, 2007·Annual Review of Microbiology·Thomas Danhorn, Clay Fuqua
Jun 11, 2014·International Journal of Phytoremediation·E KurzbaumR Armon
Apr 1, 1997·Molecular Biotechnology·J K Jansson, J I Prosser
May 26, 2001·Applied and Environmental Microbiology·G Eller, P Frenzel
Nov 29, 2014·Plant Biotechnology Journal·Kerrie FarrarNaomi Cope-Selby
Dec 7, 2005·FEMS Microbiology Ecology·Jesús Muñoz-RojasJesús Caballero-Mellado
Jan 5, 2012·PLoS Genetics·Florence Wisniewski-DyéIgor B Zhulin
Feb 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Anna V TugarovaAlexander A Kamnev
Nov 10, 2017·Current Protocols in Microbiology·Gladys Alexandre
Dec 1, 2019·World Journal of Microbiology & Biotechnology·Nina V EvseevaSergei Yu Shchyogolev

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.