In Situ Lysosomal Cysteine-Specific Targeting and Imaging during Dexamethasone-Induced Apoptosis

Analytical Chemistry
Yongkang YueCaixia Yin

Abstract

Herein we utilize the similar though divergent nucleophilic properties of cysteine, homocysteine, and glutathione to achieve the selective detection of cysteine under mildly acidic conditions. This enables the specific in situ detection of lysosomal cysteine. Employing time-dependent fluorescent imaging of probe-labeled A549 cells, we demonstrate that dexamethasone-induced apoptosis is not dependent on lysosomal cysteine. This methodology can thus produce useful information about pathogenesis associated with cysteine and lysosomes.

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Citations

Jun 18, 2019·Chemical Society Reviews·Yongkang YueCaixia Yin
Sep 12, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoli WuGuang-Ming Bao
Feb 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Songtao CaiXianshun Zeng
Jul 28, 2020·Analytical Methods : Advancing Methods and Applications·Feiran LiuXiaoling Zhang
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hemiao ZhaoCaixia Yin
Sep 2, 2020·International Journal of Molecular Sciences·Rochelin DalanginRobert E Campbell
Nov 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Weijie ZhangCaixia Yin
Feb 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yue WangMing-Yu Wu
May 18, 2021·Biochemical and Biophysical Research Communications·Guangmin LiTi Zhang
Jun 22, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yi-Hang SunCheng-Yun Wang
Jun 23, 2021·Analytical Methods : Advancing Methods and Applications·Jinlei YueWeili Zhao
Apr 4, 2020·Analytical Chemistry·Yongkang YueCaixia Yin

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Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis