In situ Microrheological Determination of Neutrophil Stiffening Following Adhesion in a Model Capillary

Annals of Biomedical Engineering
Anand PaiDavid F J Tees

Abstract

There has been considerable debate on the relative importance of biochemical stimuli and mechanical deformation in neutrophil adhesion in lung capillaries, a process observed following bacterial infection in the body. In contrast to venules, where the vessel diameter is larger than the leukocyte diameter (6-9 microm) and the adhesion process is better understood, in lung capillaries the vessel diameter (2-8 microm) is smaller than the leukocyte diameter. In this study, a micropipette was used as a model for the alveolar capillary microcirculation, allowing the effects of adhesion molecules (ICAM-1) on cell mechanical properties to be observed while applying a mechanical deformation. The microrheology technique that tracks the thermal motion of granules within neutrophils was used to extract the local intracellular viscoelastic moduli. Small regional differences in rheology were found, with the central body region being significantly stiffer than the leading end cap region. When cells were exposed to ICAM-1, the regional differences were preserved, but the viscoelastic moduli were moderately increased in all regions. These results are consistent with the literature on leukocyte sequestration and provide insight into the regional...Continue Reading

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Citations

Jun 12, 2014·Thorax·Elizabeth Sapey, Robert A Stockley
Dec 25, 2012·Biochemical and Biophysical Research Communications·Min Ye ShenHayden Huang
May 7, 2009·Annual Review of Biophysics·Denis Wirtz
Jun 28, 2008·Lab on a Chip·Michael J RosenbluthDaniel A Fletcher
Mar 6, 2019·Journal of Leukocyte Biology·Kathleen R BashantNicole Toepfner
Jun 29, 2012·Biomedical Optics Express·Jarett MichaelsonHayden Huang
Jan 10, 2020·Biology of the Cell·Kathleen R BashantEdwin R Chilvers
Apr 3, 2017·Cellular and Molecular Bioengineering·A MohammadalipourD F J Tees
Sep 30, 2020·Annals of the Rheumatic Diseases·Kathleen R BashantMariana J Kaplan

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