In situ monitoring of streptothricin production by Streptomyces rochei F20 in soil and rhizosphere

Applied and Environmental Microbiology
Usanee AnukoolElizabeth M H Wellington

Abstract

The onset of streptothricin (ST) biosynthesis in Streptomyces rochei F20 was studied by using reverse transcription-PCR (RT-PCR) to detect transcripts of ST genes during growth in liquid medium, soil, and the rhizosphere. In situ results correlated with those obtained in vitro, illustrating the growth phase-dependent manner of ST production by F20. Maximal transcription of ST resistance (sttR) and biosynthesis (sttA) genes occurred during the transition between the exponential and stationary phases of growth, when the specific growth rate (micro) started to decline. A higher level of gene expression of sttR versus sttA was observed in all experiments. In liquid culture, maximal transcript accumulation of the sttA gene was only ca. 40% that of the sttR gene. sttA and sttR mRNAs were detected in soil containing approximately 10(6) CFU of growing cells g of soil(-1). sttR mRNA was detected in sterile and nonsterile rhizosphere colonized with growing mycelium of F20 at 1.2 x 10(6) and 4.0 x 10(5) CFU g of soil(-1), respectively. However, neither sttR nor sttA transcripts were detected by RT-PCR in the rhizoplane, which supported a lower population density of F20 than the rhizosphere.

References

Nov 26, 1997·Journal of Bacteriology·M A Fernández-MorenoF Malpartida
Aug 31, 2000·Applied and Environmental Microbiology·N J BurroughsE M Wellington
May 1, 1990·Applied and Environmental Microbiology·E M WellingtonV A Saunders

❮ Previous
Next ❯

Citations

Jan 26, 2013·The Lancet Infectious Diseases·Elizabeth M H WellingtonA Prysor Williams
Feb 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sandrine DemanèchePascal Simonet
Mar 10, 2009·Applied and Environmental Microbiology·Angel Manteca, Jesus Sanchez
Jan 14, 2010·Environmental Microbiology·Paris LaskarisLiz Wellington
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ilka SchoenianDieter Spiteller
Mar 19, 2013·FEMS Microbiology Letters·Paula YagüeAngel Manteca
Sep 20, 2016·PloS One·Raies A MirKwangCheol Casey Jeong
Jan 25, 2020·Molecular Microbiology·Gleb Pishchany, Roberto Kolter
Dec 14, 2019·Acta Microbiologica Et Immunologica Hungarica·Pakhshan A Hassan, Adel K Khider

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.