In situ monitoring of the seed stage of a fermentation process using non-invasive NIR spectrometry

The Analyst
Alison NordonSarah L Stimpson

Abstract

Non-invasive NIR spectrometry has been used to monitor in situ the seed stage of a streptomyces fermentation process. The main spectral change occurred at 7263 cm(-1) in the 1st derivative spectrum, and from comparison with off-line NIR spectra acquired of components present in the fermentation broth, can be attributed to biomass. The biomass signal was constant for the first 20 h of the seed stage, after which it decreased before increasing again. The time at which the minimum occurred in the NIR profile was either the same or slightly earlier than that at which a maximum in the carbon dioxide evolution rate (CER) occurred. The changes observed for the biomass signal in the NIR spectra can be attributed to growth and then fragmentation of mycelia, which indicates a change in metabolic activity. Hence, it may be possible to use NIR spectrometry in situ to determine the optimum transfer time for the seed stage of a fermentation process. Spectra were also acquired of the final stage of the same fermentation process. The variation in the biomass signal in the NIR spectra was more complicated in the final stage owing to changes in stir rate, and biomass concentration and morphology.

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Citations

May 19, 2010·Analytical and Bioanalytical Chemistry·A S RathoreV Ghare
May 31, 2012·Analytical and Bioanalytical Chemistry·N D LourençoH M Pinheiro
Jul 24, 2010·Applied Microbiology and Biotechnology·Daniel LandgrebeKenneth F Reardon
Apr 8, 2014·Annual Review of Chemical and Biomolecular Engineering·Jay H Lee, Jong Min Lee
Sep 23, 2014·Journal of Biotechnology·José Alves-RauschDirk Bergmaier
Nov 28, 2012·Trends in Biotechnology·Andreas E PoschOliver Spadiut
Jan 9, 2016·Biotechnology Progress·Víctor Abel Corro-HerreraAnne Christine Gschaedler-Mathis
Oct 26, 2014·Applied Biochemistry and Biotechnology·Qi-Xing SunZhong-Gui Mao
Dec 3, 2016·Analytical and Bioanalytical Chemistry·Jens ClaßenThomas Scheper
Jul 2, 2017·Biotechnology and Bioengineering·Silvère AndréLudovic Duponchel

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