In situ Multispecies Colonization of An Acrylic Resin: Comparison to Oral Microbiome and Potential for Inflammatory Response

Contemporary Clinical Dentistry
António H S DelgadoJosé J Mendes

Abstract

Conventional acrylic resin is prone to microbial colonization and may cause inflammatory and allergic response. This study aims to research the initial microbial adhesion in situ and tissue response to an acrylic resin used in prosthodontics. Disks of a commercial acrylic resin were prepared and included on the surface of individual intraoral splints fabricated for 50 participants. The splints were used for 4 h, under clinical conditions. Beforehand, each participant was swabbed to provide a control for microbiological comparison. A cytological control sample was also taken from the palate. After the time elapsed, each splint was removed and growth of anaerobes, aerobes, Pseudomonas, oral streptococci, staphylococci, yeasts, and Streptococcus mutans was determined by plate counts and compared to the oral microbiome. A cytological sample was taken from the contact zone, stained using the Papanicolaou technique, analyzed in light microscopy, and classified accordingly. Means and standard deviations were calculated, and a nonparametric Wilcoxon test was employed to compare experimental groups. The significance level was set at 0.05 (95% confidence interval, and statistical analysis was performed using Statistical Package for the S...Continue Reading

Methods Mentioned

BETA
surface electron scanning microscopy

Software Mentioned

Statistical Package for the Social Sciences ( SPSS )

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