In situ photo-patterning of pressure-resistant hydrogel membranes with controlled permeabilities in PEGDA microfluidic channels

Lab on a Chip
Jérémy DecockJean-Baptiste Salmon

Abstract

We report the fabrication of highly permeable membranes in poly(ethylene glycol) diacrylate (PEGDA) channels, for investigating ultra- or micro-filtration, at the microfluidic scale. More precisely, we used a maskless UV projection setup to photo-pattern PEG-based hydrogel membranes on a large scale (mm-cm), and with a spatial resolution of a few microns. We show that these membranes can withstand trans-membrane pressure drops of up to 7 bar without any leakage, thanks to the strong anchoring of the hydrogel to the channel walls. We also report in situ measurements of the Darcy permeability of these membranes, as a function of the deposited energy during photo-polymerization, and their formulation composition. We show that the use of PEG chains as porogens, as proposed in [Lee et al., Biomacromolecules, 2010, 11, 3316], significantly increases the porosity of the hydrogels, up to Darcy permeabilities of about 1.5 × 10-16 m2, while maintaining the strong mechanical stability of the membranes. We finally illustrate the opportunities offered by this technique, by investigating frontal filtration of colloidal dispersions in a straight microfluidic channel.

References

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Citations

Mar 16, 2019·Nature Reviews. Neuroscience·Carla G SilvaLaurent Nguyen
Jun 18, 2019·Chemical Communications : Chem Comm·Stan B J WillemsVittorio Saggiomo
Jan 10, 2020·Lab on a Chip·Ulri N LeeErwin Berthier
May 1, 2020·Polymers·Alexandre Turani-I-BellotoAnd Jacques Leng
Nov 9, 2018·Biophysics Reviews·Vassilis PapalazarouLaura M Machesky
Jun 9, 2020·Lab on a Chip·Hoang-Thanh NguyenJean-Baptiste Salmon
Apr 7, 2020·Lab on a Chip·Nicolas Garcia-SeydaOlivier Theodoly
Nov 4, 2020·Membranes·Izabella Bouhid de Aguiar, Karin Schroën
Sep 18, 2021·Science Advances·Benjamin B YellenJeff Hammerbacher
Aug 31, 2021·Angewandte Chemie·Marco RoccaEmmanuel Delamarche

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