In situ plasmonic generation in functional ionic-gold-nanogel scaffold for rapid quantitative bio-sensing

Biosensors & Bioelectronics
Santosh K MisraDipanjan Pan

Abstract

Conventional analytical techniques, which have been developed for high sensitivity and selectivity for the detection and quantification of relevant biomarkers, may not be as suitable for medical diagnosis in resource scarce environments as compared to point-of-care devices (POC). We have developed a new reactive sensing material which contains ionic gold entrapped within an agarose gel scaffold for POC quantification of ascorbic acid (AA) in tear fluid. Pathologically elevated concentration of AA in human tear fluid can serve as a biomarker for full-thickness injuries to the ocular surface, which are a medical emergency. This reactive sensing material will undergo colorimetric changes, quantitatively dependent on endogenous bio-reductants that are applied, as the entrapped ionic gold is reduced to form plasmonic nanoparticles. The capacity for this reactive material to function as a plasmonically driven biosensor, called 'OjoGel' (ojo-eye), was demonstrated with the endogenous reducing agent, AA. Through applications of AA of varied concentrations to the OjoGel, we demonstrated a quantitative colorimetric relationship between red (R) hexadecimal values and concentrations of AA in said treatments. This colorimetric relationship ...Continue Reading

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Citations

Jul 31, 2020·Biosensors & Bioelectronics·Maha AlafeefDipanjan Pan
Oct 28, 2019·ACS Applied Materials & Interfaces·Maha AlafeefDipanjan Pan
Sep 28, 2021·ACS Applied Materials & Interfaces·Aaron S Schwartz-DuvalDipanjan Pan

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