In situ polymerase chain reaction-based localization studies support role of human herpesvirus-8 as the cause of two AIDS-related neoplasms: Kaposi's sarcoma and body cavity lymphoma

The Journal of Clinical Investigation
K E ForemanB J Nickoloff

Abstract

Several lines of investigation point to a new herpesvirus, human herpesvirus-8 (HHV-8), as the cause of two different neoplasms seen in AIDS patients-Kaposi's sarcoma (KS) and body cavity B cell lymphoma. If this virus is the etiological agent, rather than another opportunistic infectious agent, it should be present in the earliest detectable clinical lesions on a temporal basis, and localize to specific target cells in a spatial pattern consistent with tumorigenic pathways. In this study, we take advantage of the clinical accessibility to biopsy early (patch stage) skin lesions of KS to address the temporal issue, combined with in situ PCR and dual immunostaining using a marker identifying malignant cells, to address the spatial localization issue. 21 different tissue samples were subjected to PCR analysis and in situ PCR with and without simultaneous immunostaining. In normal skin from healthy individuals, no HHV-8 DNA was detected by PCR or in situ PCR. However, in all PCR-positive tissues, distinct and specific in situ PCR staining was observed. In four different patch stage KS lesions, in situ PCR staining localized to nuclei of endothelial cells and perivascular spindle-shaped tumor cells. Later stage KS lesions (plaques ...Continue Reading

References

Dec 1, 1995·The Journal of Infectious Diseases·L de LellisP Monini
May 4, 1995·The New England Journal of Medicine·E CesarmanD M Knowles
May 4, 1995·The New England Journal of Medicine·B Roizman
Mar 25, 1995·Lancet·Y Q HuangA E Friedman-Kien
Mar 1, 1994·Journal of Neuropathology and Experimental Neurology·P Gressens, J R Martin
Jan 3, 1996·International Journal of Cancer. Journal International Du Cancer·F M BuonaguroG Giraldo
May 2, 1996·The New England Journal of Medicine·P MoniniE Cassai
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·W ZhongD Ganem
Aug 1, 1996·Nature Medicine·J J O'Leary
Aug 17, 1996·Lancet·C A SanderP Kind
Jan 16, 1997·The New England Journal of Medicine·K E ForemanG J Nabel

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Citations

Jun 9, 2001·International Journal of Pharmaceutics·H WuB J Roessler
Jun 8, 2001·Journal of Dermatological Science·K E ForemanB J Nickoloff
Oct 12, 2000·The Journal of Infection·T F Schulz
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·N DupinC Boshoff
Jul 28, 2006·The American Journal of Dermatopathology·Jean L UrquhartSabine Kohler
Dec 6, 2003·The American Journal of Surgical Pathology·Erich J SchwartzSabine Kohler
Apr 21, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B Damania, R C Desrosiers
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Lyubomir A DourmishevDavid M Lukac
Jul 5, 2005·Expert Opinion on Investigational Drugs·K J KallenS Rose-John
Dec 20, 2002·Clinics in Laboratory Medicine·Monica Gandhi, Ruth M Greenblatt
Nov 7, 1999·Periodontology 2000·C B Fowler
Jul 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·J J Gómez-RománJ F Val-Bernal
Jun 13, 2001·Journal of Cutaneous Pathology·M Nuovo, G Nuovo
Feb 11, 1999·Journal of Virology·R SunG Miller
Jun 15, 2000·The American Journal of Pathology·M MercaderK E Foreman
Nov 8, 2002·Biochimica Et Biophysica Acta·Karl-Josef Kallen
Mar 7, 2001·Archives of Pathology & Laboratory Medicine·H HoffmannL J Medeiros
May 24, 2001·Archives of Pathology & Laboratory Medicine·M MercaderK E Foreman
Dec 3, 1999·Archives of Pathology & Laboratory Medicine·J J Gómez-RománJ F Val-Bernal

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