In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications

Scientific Reports
Virginie KeriquelJean-Christophe Fricain

Abstract

Bioprinting has emerged as a novel technological approach with the potential to address unsolved questions in the field of tissue engineering. We have recently shown that Laser Assisted Bioprinting (LAB), due to its unprecedented cell printing resolution and precision, is an attractive tool for the in situ printing of a bone substitute. Here, we show that LAB can be used for the in situ printing of mesenchymal stromal cells, associated with collagen and nano-hydroxyapatite, in order to favor bone regeneration, in a calvaria defect model in mice. Also, by testing different cell printing geometries, we show that different cellular arrangements impact on bone tissue regeneration. This work opens new avenues on the development of novel strategies, using in situ bioprinting, for the building of tissues, from the ground up.

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Methods Mentioned

BETA
bioprintng
Bioprinting
Bioprinters
X-ray
bioprinter
FCS

Software Mentioned

Photo
3D Vision
Graphpad Prism
Microview®
eXplore MicroView®
- acquisition

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