Abstract
Saccharomyces cerevisiae immobilized in agarose gel as binding phase and polyacrylamide as diffusive layer in the diffusive gradient in thin films technique (DGT) was used for selective determination of methylmercury (MeHg). Deployment tests showed good linearity in mass uptake up to 48 h (3276 ng). When coupling the DGT technique with Cold Vapor Atomic Fluorescence Spectrometry, the method has a limit of detection of 0.44 ng L(-1) (pre concentration factor of 11 for 48 h deployment). Diffusion coefficient of 7.03 ± 0.77 × 10(-6) cm(2) s(-1) at 23 °C in polyacrylamide gel (pH = 5.5 and ionic strength = 0.05 mol L(-1) NaCl) was obtained. Influence of ionic strength (from 0.0005 mol L(-1) to 0.1 mol L(-1) NaCl) and pH (from 3.5 to 8.5) on MeHg uptake were evaluated. For these range, recoveries of 84-105% and 84-98% were obtained for ionic strength and pH respectively. Potential interference due to presence of Cu, Fe, Mn, Zn was also assessed showing good recoveries (70-87%). The selectivity of the proposed approach was tested by deployments in solutions containing MeHg and Hg(II). Results obtained showed recoveries of 102-115 % for MeHg, while the uptake of Hg(II) was insignificant. The proposed approach was successfully employed...Continue Reading
References
Feb 15, 1995·Analytical Chemistry·Y MadridC Cámara
Aug 3, 2001·The Science of the Total Environment·P S Fadini, W F Jardim
Jan 1, 2005·The Science of the Total Environment·Jennifer L Parker, Nicolas S Bloom
Feb 19, 2005·Environmental Geochemistry and Health·Mark C Gabriel, Derek G Williamson
Nov 30, 2006·Journal of Environmental Monitoring : JEM·Olivier Clarisse, Holger Hintelmann
Oct 31, 2008·Talanta·H Docekalová, P Divis
Nov 26, 2008·Environmental Pollution·Olivier ClarisseHolger Hintelmann
Nov 26, 2010·Analytica Chimica Acta·Amauri A MenegárioSteven F Durrant
Jun 17, 2011·Environmental Science & Technology·Yong Seok HongEdward J Bouwer
Sep 20, 2011·Chemosphere·C Fernández-GómezS Díez
Nov 22, 2011·Talanta·Yue GaoPascal Van Der Voort
Jan 10, 2012·The Science of the Total Environment·O ClarisseE P H Best
Jul 21, 2012·Analytical and Bioanalytical Chemistry·Guilherme Favoreto PescimAmauri Antonio Menegário
Sep 21, 2013·Environmental Monitoring and Assessment·Lauren Nozomi Marques YabukiDomitila Pascoaloto
Jan 29, 2014·Talanta·Yue GaoWilly Baeyens
Mar 19, 2014·Analytica Chimica Acta·Pavlína PelcováAndrea Kleckerová
Jul 11, 2014·The Analyst·Ana M C M RolisolaRicardo E Santelli
Aug 17, 2014·Talanta·Camila Destro ColaçoVirgílio Franco do Nascimento Filho
Aug 17, 2014·Talanta·Cristal Fernández-GómezSergi Díez