In situ stable isotope probing of phosphate-solubilizing bacteria in the hyphosphere

Journal of Experimental Botany
Fei WangGu Feng

Abstract

This study used a [(13)C]DNA stable isotope probing (SIP) technique to elucidate a direct pathway for the translocation of (13)C-labeled photoassimilate from maize plants to extraradical mycelium-associated phosphate-solubilizing bacteria (PSB) that mediate the mineralization and turnover of soil organic phosphorus (P) in the hyphosphere. Inoculation with PSB alone did not provide any benefit to maize plants but utilized the added phytate-P to their own advantage, while inoculation with Rhizophagus irregularis alone significantly promoted shoot biomass and P content compared with the control. However, compared with both sole inoculation treatments, combined inoculation with PSB and R. irregularis in the hyphosphere enhanced organic P mineralization and increased microbial biomass P in the soil. There was no extra benefit to plant P uptake but the hyphal growth of R. irregularis was reduced, suggesting that PSB benefited from the arbuscular mycorrhizal (AM) fungal mycelium and competed for soil P with the fungus. The combination of T-RFLP (terminal restriction fragment length polymorphism) analysis with a clone library revealed that one of the bacteria that actively assimilated carbon derived from pulse-labeled maize plants was ...Continue Reading

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Citations

Jul 27, 2017·Frontiers in Microbiology·Ubiana C SilvaVera L Dos Santos
Feb 6, 2019·Plant, Cell & Environment·Jon R HelliwellSacha J Mooney
Apr 13, 2021·Frontiers in Microbiology·Mon Oo YeeRomy Chakraborty

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