In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria

ACS Omega
Upashi GoswamiSiddhartha Sankar Ghosh

Abstract

Herein, we introduce a new facile method of luminescent gold nanocluster (Au NC) synthesis on the surface of bacteria for detection, counting, and strain differentiation. The limit of detection was 740 ± 14 colony-forming unit (CFU)/mL for the Gram-negative and was 634 ± 16 CFU/mL for the Gram-positive bacteria. Brief treatment with lysozyme could differentiate the Gram strains based on their luminescence intensities. The current method could also detect bacterial contaminants from water sources and kanamycin-resistant strains rapidly. This quick synthesis of Au NCs on a bacterial template attributes an easy and rapid method for enumeration and detection of bacterial contaminants and kanamycin-resistant strains.

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Citations

Nov 9, 2019·Nanotechnology·Arushi VermaMd Palashuddin Sk
Mar 25, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zengchao GuoXuemei Wang
Nov 21, 2019·Nanoscale·Dan LiVincent Rotello
Jul 31, 2020·Biosensors & Bioelectronics·Maha AlafeefDipanjan Pan
Jul 21, 2021·Critical Reviews in Food Science and Nutrition·Yi XuQuansheng Chen

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Methods Mentioned

BETA
X-ray
AFM
atomic force microscopy

Software Mentioned

Gwyddion

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