In situ tight-seal recordings of taste substance-elicited action currents and voltage-gated Ba currents from single taste bud cells in the peeled epithelium of mouse tongue

Brain Research
H Furue, Kiyonori Yoshii

Abstract

We investigated the taste responses of single taste-bud cells (TBCs) in mice by applying stimuli only on receptor membranes acclimated to deionized water under tight-seal cell-attached voltage-clamp conditions, while their basolateral membranes were irrigated with a physiological saline solution. For this irrigation, we developed a new method: a peeled-tongue epithelium with TBCs mounted on a recording chamber where the peeled epithelium separated the irrigating solutions for each membrane as it separated in situ. Although no quinine-elicited action potentials had been reported, TBCs elicited a long-lasting train of biphasic currents derived from the action potentials in response to 10 mM quinine, in addition to responses to 10 mM HCl, or 200 mM NaCl dissolved in deionized water. These results indicate that quinine as well as HCl and NaCl depolarizes TBCs and generate action potentials. Under whole-cell recording conditions, TBCs generated action potentials, and voltage-gated currents such as LVA and HVA Ca currents, TTX-sensitive Na currents, and TEA/4-AP-sensitive K currents on depolarization. These voltage-gated channels were shown to exist predominantly on the basolateral membranes. We discussed the receptor mechanisms and ...Continue Reading

Citations

Mar 17, 2012·Pflügers Archiv : European journal of physiology·Namie BeppuKiyonori Yoshii
Jan 9, 2020·The European Journal of Neuroscience·Masafumi IwamotoYoshitaka Ohtubo
Mar 29, 2020·Sensors·Elena von MolitorTiziana Cesetti
Apr 5, 2001·Journal of Neurophysiology·A Bigiani
Feb 9, 2007·Archives of Histology and Cytology·Ryusuke YoshidaYuzo Ninomiya
Feb 24, 2001·The Journal of Physiology·Y OhtuboK Y Yoshii
Sep 18, 2010·Head & Neck·Helga Susanne HaasAnton Sadjak
Apr 3, 2012·The European Journal of Neuroscience·Yoshitaka OhtuboKiyonori Yoshii
Sep 15, 2006·Journal of Neurophysiology·Ryusuke YoshidaYuzo Ninomiya
Feb 17, 2017·Journal of Neurophysiology·Zhongming MaJ Kevin Foskett
Jul 26, 2003·The Journal of Physiology·Yoko HigureKiyonori Yoshii
Nov 26, 2009·Science in China. Series C, Life Sciences·PeiHua ChenJianHong Luo
Oct 25, 2013·The European Journal of Neuroscience·Kenji KimuraKiyonori Yoshii
Nov 12, 2020·Experimental Physiology·Keita TakeuchiYoshitaka Ohtubo
Jul 31, 2003·Progress in Biophysics and Molecular Biology·Albertino BigianiFrancesca Fieni
Apr 22, 2021·Pflügers Archiv : European journal of physiology·Yoshitaka Ohtubo
Jun 1, 2000·Progress in Neurobiology·T MiyamotoT Sato
Apr 12, 2002·Progress in Neurobiology·Albertino Bigiani
Mar 17, 2009·BMC Neuroscience·Na GaoBryan D Moyer

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