In situ topology of cytochrome b5 in the endoplasmic reticulum membrane

Journal of Biochemistry
R KurodaA Ito

Abstract

Cytochrome b5 is tail-anchored in the ER membrane and is composed of three functionally different portions; amino-terminal heme-containing catalytic, central hydrophobic membrane-anchoring, and carboxy-terminal ER-targeting portions [Mitoma, J. and Ito, A. (1992) EMBO J. 11, 4197-4203]. In situ topology of cytochrome b5 in the ER-membrane was studied using immunofluorescence microscopy. Antibodies were raised against the hydrophilic portion (anti-b5) and the carboxy-terminal seven amino acid residues (anti-peptide) of cytochrome b5 and used for detection of the cytochrome in COS cells which expressed the rat cytochrome. Anti-b5 antibody detected the cytochrome in a reticular staining pattern characteristic of the ER, even when the cell plasma membrane was permeabilized with Streptolysin O. The anti-peptide displayed a fluorescence signal only with Triton-permeabilized cells in which the antibody was able to penetrate into the ER lumen. In a double immuno-staining of the cell using the antipeptide antibody and the antibody against protein disulfide isomerase, both antibodies showed the same staining pattern in the presence of either Triton X-100 or Streptolysin O. The results indicate that the carboxy-terminal hydrophilic stretc...Continue Reading

Citations

Dec 30, 2009·Cellular and Molecular Life Sciences : CMLS·Robert Renthal
Jan 22, 2004·Biochemical and Biophysical Research Communications·Adriana AltuveMario Rivera
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M GollubL Shaw
Nov 4, 2000·The Biochemical Journal·M R HanlonB A Wallace
Apr 7, 2010·Journal of Biomedicine & Biotechnology·Salvador Eugenio C Caoili
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·H ZhuH F Bunn
Feb 16, 2016·FEBS Letters·Veronika ZámbóÉva Kereszturi
Mar 27, 2007·Biochimica Et Biophysica Acta·Thomas Strahl, Jeremy Thorner
Jun 23, 2009·BMC Cell Biology·Joseph T ByersBalwant S Tuana
Oct 3, 1999·The Journal of Biological Chemistry·D L GalvanG A Mignery
Jan 27, 2005·The Journal of Biological Chemistry·Richard S Stewart, David A Harris
Mar 27, 1998·Archives of Biochemistry and Biophysics·Y KidaM Sakaguchi

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