In-source decay characteristics of peptides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Journal of the American Society for Mass Spectrometry
M Takayama

Abstract

In-source decay (ISD) of peptides, coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, has been examined to determine the influence of the matrix, the susceptibility of amino-acid residues to ISD, and the effect of extraction delay times. Out of nine di- and tri-hydroxybenzoic acids and three cinnamic derivatives tested, the most suitable matrix for ISD was 2,5-dihydroxybenzoic acid. The amine bond at Xxx-Gly and Xxx-Val residues was less susceptible than other amino-acid residues to ISD; however, the more sensitive residue(s) were not as clear. Using a peptide that gave the y(n)- and (z(n) + 2)-series product ions, it was confirmed that amide-bond cleavage (formation of the y(n)-series ions) accompanied metastable peaks, whereas metastable peaks were never observed with amine-bond cleavage [formation of the (z(n) + 2)-series ions]. Furthermore, abundant c(n)-series ions, which originate from amine-bond cleavage on the peptide backbone, were observed whenever a minimum delay time of 38 ns or continuous extraction was used to obtain spectra. These data indicate that amine-bond cleavage in ISD takes place on the ionization time scale before the energy randomization is completed.

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Citations

Mar 29, 2005·Journal of the American Society for Mass Spectrometry·Zhaoyang Liu, Kevin L Schey
Jan 6, 2007·Journal of the American Society for Mass Spectrometry·Jennifer M CampbellAlfred L Yergey
Nov 21, 2008·Journal of the American Society for Mass Spectrometry·Chul YooMinhui Ma
Sep 14, 2010·Journal of the American Society for Mass Spectrometry·Kevin DemeureEdwin Andre De Pauw
Sep 1, 2009·Bioanalysis·Marcus Macht
Dec 21, 2011·Journal of the American Society for Mass Spectrometry·Nicolas SmargiassoEdwin De Pauw
Jan 12, 2012·The Journal of Biological Chemistry·Tadashi NakaiToshihide Okajima
Jul 20, 2010·Annual Review of Analytical Chemistry·Mélanie AbonnencHubert H Girault
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Daiki Asakawa, Mitsuo Takayama
Feb 13, 2016·Journal of the American Society for Mass Spectrometry·Chelsea L McMillenCarolyn J Cassady
Apr 12, 2011·Journal of Proteomics·David CalligarisDaniel Lafitte
Jul 1, 2005·Rapid Communications in Mass Spectrometry : RCM·R SrikanthPalakodety Radha Krishna
Jun 6, 2009·Chemistry : a European Journal·Liang QiaoBaohong Liu
Nov 10, 2006·Journal of Mass Spectrometry : JMS·D DeboisO Laprévote
Jun 24, 2011·Rapid Communications in Mass Spectrometry : RCM·Julie HardouinAgnès F Delmas
May 11, 2016·Mass Spectrometry·Mitsuo Takayama
Feb 4, 2006·Rapid Communications in Mass Spectrometry : RCM·Julie HardouinCatherine Lange
May 5, 2016·Journal of the American Society for Mass Spectrometry·Jean E BoulicaultRichard B Cole
Jun 22, 2016·Journal of Pharmaceutical and Biomedical Analysis·Maria Kristina ParrHouda Montacir
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Oct 30, 2010·Analytical Chemistry·Nicolas Smargiasso, Edwin De Pauw

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