In the absence of endogenous mouse apolipoprotein E, apolipoprotein E*2(Arg-158 --> Cys) transgenic mice develop more severe hyperlipoproteinemia than apolipoprotein E*3-Leiden transgenic mice.

The Journal of Biological Chemistry
B J van VlijmenL M Havekes

Abstract

Apolipoprotein E*2(Arg-158 --> Cys) (APOE*2) transgenic mice were generated and compared to the previously generated apolipoprotein E*3-Leiden (APOE*3-Leiden) transgenic mice to study the variable expression of hyperlipoproteinemia associated with these two APOE variants. In the presence of the endogenous mouse Apoe gene, the expression of the APOE*3-Leiden gene resulted in slightly elevated levels of serum cholesterol as compared with control mice (2.7 +/- 0. 5 versus 2.1 +/- 0.2 mmol/liter, respectively), whereas the expression of the APOE*2(Arg-158 --> Cys) gene did not affect serum cholesterol levels, even after high/fat cholesterol feeding. The extreme cholesterol level usually found in apoE-deficient mice (Apoe-/- mice; 23.6 +/- 5.0 mmol/liter) could be rescued by introducing the APOE*3-Leiden gene (APOE*3-Leiden.Apoe-/-; 3.6 +/- 1. 5 mmol/liter), whereas the expression of the APOE*2(Arg-158 --> Cys) gene in Apoe-/- mice minimally reduced serum cholesterol levels (APOE*2.Apoe-/-; 16.6 +/- 2.9 mmol/liter). In vivo very low density lipoprotein (VLDL) turnover studies revealed that APOE*2.Apoe-/- VLDL and APOE*3-Leiden.Apoe-/- VLDL display strongly reduced fractional catabolic rates as compared with control mouse VLDL (4.0 a...Continue Reading

References

May 12, 1975·Analytical Biochemistry·T G RedgraveC E West
Jun 11, 1992·Journal of Internal Medicine·S C Rall, R W Mahley
Feb 5, 1991·Biochimica Et Biophysica Acta·M MulderL M Havekes
Nov 1, 1991·The Journal of Clinical Investigation·T DemantJ Shepherd
Nov 1, 1986·The Journal of Clinical Investigation·E J SchaeferH B Brewer
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C EhnholmJ L Witztum
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T KitaJ L Goldstein
Apr 1, 1994·The Journal of Clinical Investigation·B J van VlijmenL M Havekes

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Citations

May 19, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Kristiaan WoutersMarten H Hofker
Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·J DavignonL Bernier
Jan 14, 2003·Seminars in Cell & Developmental Biology·Peter Marschang, Joachim Herz
Aug 11, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·A R MensenkampF Kuipers
Dec 11, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·K W van DijkM H Hofker
Sep 2, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Marit WesterterpPatrick C N Rensen
Mar 30, 2001·The Journal of Biological Chemistry·A D TagalakisJ S Owen
Mar 12, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·F de BeerL M Havekes
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K E KypreosV I Zannis
Oct 29, 1998·Biological Chemistry·M FoianiP Plevani
Oct 24, 1998·Endocrinology and Metabolism Clinics of North America·J M Hoeg

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