In-trap cleanup of proteins from electrospray ionization using soft sustained off-resonance irradiation with fourier transform ion cyclotron resonance mass spectrometry

Analytical Chemistry
L P TolićR D Smith

Abstract

Electrospray ionization Fourier transform ion cyclotron resonance (FTICR) mass spectrometry is capable of producing high mass resolving power and improved mass accuracy for large proteins and noncovalent complexes when coupled with collisionally induced dissociation (CID) of noncovalent adducts and consequent minimization of ion charge density in the ICR trap during measurements. This work demonstrates the application of in-trap cleanup to several biologically relevant systems, including carbonic anhydrase, 4-oxalocrotonate tautomerase (4OT) analogue, and SecB, a chaperone from Escherichia coli. In-trap cleanup yields improved mass measurements for these systems and is expected to further enable measurements for even more complex systems where adduction levels have precluded study of intact complexes.

References

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Citations

Feb 27, 2001·Mass Spectrometry Reviews·J Godovac-Zimmermann, L R Brown
May 30, 1998·Protein Science : a Publication of the Protein Society·J E BruceR D Smith
Nov 25, 1998·Protein Science : a Publication of the Protein Society·L L RandallR D Smith
Sep 18, 2013·Journal of the American Society for Mass Spectrometry·Feng XianAlan G Marshall
Dec 27, 2008·Journal of the American Society for Mass Spectrometry·Justin L P Benesch
Feb 27, 1999·Current Opinion in Biotechnology·C E Costello
Oct 6, 1999·Current Opinion in Chemical Biology·A M Last, C V Robinson
Feb 10, 2012·Journal of the Royal Society, Interface·Gillian R Hilton, Justin L P Benesch
Apr 5, 2014·Journal of the American Society for Mass Spectrometry·Philip LösslAlbert J R Heck
Jan 23, 2013·FEBS Letters·Hao ZhangRobert E Blankenship
Sep 25, 2004·Mass Spectrometry Reviews·Bogdan Bogdanov, Richard D Smith
Sep 25, 2004·Mass Spectrometry Reviews·Julia Laskin, Jean H Futrell
Apr 11, 2000·Journal of the American Society for Mass Spectrometry·D S WunschelR D Smith
Dec 18, 2010·Analytical Chemistry·Yongjing ChenPurnendu K Dasgupta

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