In utero and lactational exposures to diethylhexyl-phthalate affect two populations of Leydig cells in male Long-Evans rats.

Biology of Reproduction
Han LinRen-Shan Ge

Abstract

Diethylhexylphthalate (DEHP) has been classified as an antiandrogen. However, whether in utero and lactational exposures of DEHP affect Leydig cells has not been well established. In the present study, the effects of DEHP exposures on fetal Leydig cells (FLCs) and adult Leydig cells (ALCs) were assessed. Pregnant dams of Long-Evans rats were treated with 0, 10, and 750 mg/kg body weight DEHP from Gestational Day 12.5 to Postnatal Day (PND) 21.5. Fetal Leydig cell clustering and FLC-specific gene expression were examined. Anogenital distances (AGDs) of male pups were assessed at PND 2. Serum testosterone levels of male pups and mRNA levels of ALC-specific genes were measured at PNDs 21 and 49. The AGDs of male pups were significantly shorter in the group treated with 750 mg/kg DEHP (mean +/- SEM, 3.68 +/- 0.16 mm) compared with control (4.62 +/- 0.13 mm). The FLCs were aggregated after 10 and 750 mg/kg DEHP exposures. Several FLC-specific genes, including luteinizing hormone receptor (Lhcgr) and steroidogenic enzyme genes, were downregulated at both doses. Serum testosterone levels were significantly lower compared with control at PND 21 after treatment of 10 or 750 mg/kg DEHP, and continued to be lower even up to 49 days postpa...Continue Reading

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Citations

Apr 2, 2013·Analytica Chimica Acta·Xiaodong XiaJianxiu Wang
Nov 28, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·D N Rao Veeramachaneni, Gary R Klinefelter
Apr 17, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Jingjing GuoRen-Shan Ge
Jan 30, 2014·Environmental Science and Pollution Research International·Chunhua LiuXia Huo
Nov 1, 2015·International Journal of Environmental Research and Public Health·Han LinRen-Shan Ge
May 9, 2014·International Journal of Molecular Sciences·Bingbing ChenXiaokun Li
Mar 13, 2009·Trends in Endocrinology and Metabolism : TEM·Guo-Xin HuXiao-Kun Li
Feb 26, 2016·International Journal of Environmental Research and Public Health·Xiaoheng LiRen-Shan Ge
Dec 21, 2017·Journal of Molecular Endocrinology·Yiyan WangRen-Shan Ge
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May 23, 2020·Journal of Cellular and Molecular Medicine·Xiaoheng LiYadong Huang
Apr 3, 2021·Toxicology and Applied Pharmacology·Haiqiong ChenRen-Shan Ge

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GraphPad Prism
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