In utero exposure to benzene disrupts fetal hematopoietic progenitor cell growth via reactive oxygen species

Toxicological Sciences : an Official Journal of the Society of Toxicology
Helen J Badham, Louise M Winn

Abstract

It is hypothesized that the increasing incidence of childhood leukemia may be due to in utero exposure to environmental pollutants, such as benzene, but the mechanisms involved remain unknown. We hypothesize that reactive oxygen species (ROS) contribute to the deregulation of fetal hematopoiesis caused by in utero benzene exposure. To evaluate this hypothesis, pregnant C57Bl/6N mice were exposed to benzene or polyethylene glycol-conjugated catalase (PEG-catalase) (antioxidative enzyme) and benzene. Colony formation assays on fetal liver cells were performed to measure erythroid and myeloid progenitor cell growth potential. The presence of ROS in CD117(+) fetal liver cells was measured by flow cytometric analysis. Oxidative cellular damage was assessed by Western blot analysis of 4-hydroxynonenol (4-HNE) and nitrotyrosine products, as well as reduced to oxidized glutathione ratios. Alterations in the redox-sensitive signaling pathway nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-kappaB) were measured by Western blot analysis of Inhibitor of NF-kB-alpha (IkappaB-alpha) protein levels in fetal liver tissue. In utero exposure to benzene caused a significant increase in ROS production and significantly altered f...Continue Reading

References

Sep 15, 1984·Toxicology and Applied Pharmacology·E P CronkiteR T Drew
May 1, 1984·Toxicology and Applied Pharmacology·K E Driscoll, C A Snyder
Mar 10, 1995·Archives of Biochemistry and Biophysics·D A StoyanovskyV E Kagan
Dec 1, 1993·Carcinogenesis·P M SchlosserM A Medinsky
Aug 1, 1996·Cancer·J G GurneyL L Robison
Feb 1, 1997·Biochemical Society Transactions·A Bowie, L A O'Neill
Dec 1, 1996·Environmental Health Perspectives·R F Henderson
Jan 1, 1997·International Archives of Occupational and Environmental Health·R Nordlinder, B Järvholm
Jan 23, 1999·Free Radical Biology & Medicine·L M Winn, P G Wells
May 2, 2002·Cell·Sankar Ghosh, Michael Karin
Dec 30, 2003·International Journal of Cancer. Journal International Du Cancer·Paolo CrosignaniFranco Berrino
Feb 3, 2004·Oncogene·Thomas D GilmoreDaniel T Starczynowski
Jun 5, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ann Charlotte DreifaldtLennart Hardell
Sep 17, 2004·Genes & Development·Matthew S Hayden, Sankar Ghosh
Dec 4, 2004·Science·Qing LanMartyn T Smith
May 4, 2005·Current Opinion in Hematology·Elaine Dzierzak
Aug 6, 2005·Toxicology and Applied Pharmacology·Peter G WellsAndrea W Wong
Sep 6, 2005·Chemico-biological Interactions·Kun-Ming ChenAssieh A Melikian
Jan 25, 2006·Chemico-biological Interactions·M ValkoM Mazur
Jul 11, 2006·Journal of Neuropathology and Experimental Neurology·Akihiko NunomuraMark A Smith
Feb 23, 2007·Birth Defects Research. Part C, Embryo Today : Reviews·Joanne Wan, Louise M Winn
Feb 19, 2008·Toxicology and Applied Pharmacology·Joanne Wan, Louise M Winn
Jan 8, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Peter G WellsAndrea W Wong

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Citations

Nov 12, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Samantha E Stokes, Louise M Winn
May 1, 2013·Mutation Research·Angela M MoroSolange C Garcia
Mar 19, 2011·Toxicology Letters·Xiao-Rong WuZong-Chun Yi
Nov 22, 2015·American Journal of Epidemiology·Frolayne M Carlos-WallaceCraig Steinmaus
Jan 15, 2014·ISRN Toxicology·Priyanka MandaniHyacinth Highland
Nov 17, 2009·Chemico-biological Interactions·Helen J BadhamLouise M Winn
Sep 29, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Lingli HuangZonghui Yuan
Sep 6, 2015·Toxicology and Applied Pharmacology·Nicola A Philbrook, Louise M Winn
Feb 28, 2014·Annals of the New York Academy of Sciences·Cliona M McHaleLuoping Zhang
May 15, 2015·Journal of Agricultural and Food Chemistry·Huang LingliYuan Zonghui
Mar 13, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Kristin M JungeIrina Lehmann
Apr 15, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Juan LiZonghui Yuan
Mar 1, 2020·Journal of Applied Toxicology : JAT·Peter C W LuLouise M Winn
Dec 20, 2019·BMC Pharmacology & Toxicology·Angela Maria MoroSolange Cristina Garcia
Jul 25, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Trent H Holmes, Louise M Winn
Oct 13, 2015·Journal of Applied Toxicology : JAT·Nicola A Philbrook, Louise M Winn
Sep 9, 2017·PloS One·Wesley AbplanalpTimothy E O'Toole
May 20, 2021·Environmental Science and Pollution Research International·Tong WeiFen Liu

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