In utero exposure to di-(2-ethylhexyl) phthalate induces testicular effects in neonatal rats that are antagonized by genistein cotreatment

Biology of Reproduction
Steven JonesM Culty

Abstract

Fetal exposure to endocrine disruptors (EDs) is believed to predispose males to reproductive abnormalities. Although males are exposed to combinations of chemicals, few studies have evaluated the effects of ED mixtures at environmentally relevant doses. Our previous work showed that fetal exposure to a mixture of the phytoestrogen genistein (GEN) and the plasticizer di-(2-ethylhexyl) phthalate (DEHP) induced unique alterations in adult testis. In this follow-up study, we examined Postnatal Day 3 (PND3) and PND6 male offspring exposed from Gestational Day 14 to parturition to corn oil, 10mg/kg GEN, DEHP, or their combination, to gain insight into the early molecular events driving long-term alterations. DEHP stimulated the mRNA and protein expression of the steroidogenic enzyme HSD3B, uniquely at PND3. DEHP also increased the mRNA expression of Nestin, a Leydig progenitor/Sertoli cell marker, and markers of Sertoli cell (Wt1), gonocyte (Plzf, Foxo1), and proliferation (Pcna) at PND3, while these genes were unchanged by the mixture. Redox (Nqo1, Sod2, Sod3, Trx, Gst, Cat) and xenobiotic transporter (Abcb1b, Abcg2) gene expression was also increased by DEHP at PND3, while attenuated when combined with GEN, suggesting the involveme...Continue Reading

References

Sep 12, 1992·BMJ : British Medical Journal·E CarlsenN E Skakkebaek
Dec 1, 1981·Journal of Steroid Biochemistry·G L GunsalusC W Bardin
May 1, 1993·The Journal of Cell Biology·S Citi
Oct 1, 1995·Environmental Health Perspectives·E CarlsenN E Skakkebaek
May 1, 1997·Differentiation; Research in Biological Diversity·K FröjdmanJ E Eriksson
Dec 3, 1999·Toxicology and Applied Pharmacology·E K Maloney, D J Waxman
Mar 13, 2002·Toxicology Letters·Warren G FosterClaude L Hughes
Nov 22, 2002·Biology of Reproduction·Nathalie MelaineBernard Jégou
Mar 1, 1957·The American Journal of Anatomy·Y CLERMONT, B PEREY
Apr 10, 2004·Journal of Molecular Endocrinology·S R KingM E Reyland
Apr 15, 2004·Reproductive Toxicology·Céline FioriniGeorges Pointis
Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antonia M CalafatLarry L Needham
Sep 3, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J BartW T A van der Graaf
Sep 15, 2004·Bulletin of Environmental Contamination and Toxicology·M J SilvaA M Calafat
Dec 1, 2004·The Journal of Cell Biology·Michail S DavidoffDieter Müller
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Helen H N Yan, C Yan Cheng
Nov 2, 2005·Biochimica Et Biophysica Acta·Georges PointisDominique Segretain
Jan 31, 2006·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Hendrina A de Boo, Jane E Harding
Mar 9, 2006·Best Practice & Research. Clinical Endocrinology & Metabolism·Richard M Sharpe
May 9, 2006·Reproductive Toxicology·Cristian M SobarzoBerta Denduchis
Dec 23, 2006·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Karl K RozmanDavid Umbach
Jun 26, 2007·Fertility and Sterility·Tina K JensenNiels Erik Skakkebaek
Jul 12, 2007·Nature Clinical Practice. Urology·Ulla N JoensenNiels E Skakkebaek
Jul 24, 2007·Molecular Pharmacology·Junichi EnokizonoYuichi Sugiyama
Oct 19, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shannon Reagan-ShawNihal Ahmad
Jan 17, 2008·Nature Reviews. Genetics·Hiroyuki Sasaki, Yasuhisa Matsui
Jan 30, 2008·Basic & Clinical Pharmacology & Toxicology·Ulla Nordström JoensenNiels Erik Skakkebaek
Feb 29, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·David GunnarssonGunnar Selstam
Mar 28, 2008·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Karen P Phillips, Nongnuj Tanphaichitr
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·R M Sharpe
Jan 24, 2009·Environmental Health Perspectives·Romain LambrotVirginie Rouiller-Fabre
Mar 24, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Martine Culty

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Citations

Sep 11, 2018·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·David C DormanKatrina M Waters
Nov 27, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daniel J SpadeKim Boekelheide
Sep 13, 2017·Scientific Reports·Enrico CampioliVassilios Papadopoulos
Apr 3, 2020·Environmental Science and Pollution Research International·Aylin BalciBelma Kocer-Gumusel
Jun 23, 2020·Oxidative Medicine and Cellular Longevity·Tong-Dian ZhangLian-Dong Zhang
Oct 14, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Julio BuñayRicardo D Moreno

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