PMID: 6983024Jan 1, 1982Paper

In vitro action of 1,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol on matrix organization and mineral distribution in rabbit growth plate

Metabolic Bone Disease & Related Research
J J PlachotS Balsan

Abstract

Growth plates of 18-day-old rabbits were incubated in a protein-free synthetic medium, either without any additive, with 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] (10(-10) M), with 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] (10(-10) M and 10(-9) M), with both metabolites, or with the ethanol solvent alone. Cartilages, before and after 5 days of incubation, were studied by light and electron microscopy. The intracellular calcium distribution was analyzed by the potassium pyroantimonate method, and the calcium content was verified by x-ray microprobe analysis. When compared to nonincubated samples the cartilages incubated for 5 days without any additive as well as the cartilages incubated with the solvent alone showed excessive hydratation and hypertrophy of the chondrocytes, which had lost their columnar arrangement. The matrix and the cells were devoid of mineral. The ultrastructure of the cells was well preserved. These changes were largely prevented by the presence of both vitamin D3 metabolites. With regard to calcium distribution, 1,25-(OH)2D3 maintained calcium in mitochondria and crystals in matrix vesicles, whereas 24,25-(OH)2D3 only partly maintained mitochondrial mineral. In the chondrocytes incubated with this ...Continue Reading

References

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Citations

Sep 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B GrosseM Lieberherr
Sep 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C FarquharsonN Loveridge
May 1, 2012·Current Osteoporosis Reports·Daniel D Bikle
Nov 15, 2002·Molecular and Cellular Endocrinology·H A W Hazewinkel, M A Tryfonidou
Apr 17, 2001·The Journal of Small Animal Practice·F M Harcourt-Brown, S J Baker
Feb 18, 2014·Chemistry & Biology·Daniel D Bikle
Dec 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L V HaleR E Wuthier
Jan 1, 1983·Metabolic Bone Disease & Related Research·H K VäänänenH C Anderson
Aug 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A Hinek, A R Poole
May 1, 1987·Journal of Dairy Science·T A Reinhardt, F G Hustmyer
Feb 8, 2020·Nature Reviews. Endocrinology·Daniel Bikle, Sylvia Christakos
Jan 1, 1992·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B D BoyanL D Swain
Dec 9, 2020·Metabolites·Rafael Vessecchi Amorim ZafalonMarcio Antonio Brunetto
Dec 22, 2020·The Clinical Biochemist. Reviews·Indra Ramasamy
Jul 13, 1983·Biochimica Et Biophysica Acta·A EdelmanS Balsan
Jun 29, 1983·Biochemical and Biophysical Research Communications·G KidroniM Ron

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