Abstract
Complement activation occurs secondary to a variety of external stimuli. Lactic acidosis has been previously shown to activate the complement factors C3a and C5a. In the present investigation we examined the differential effect of lactic acidosis on anaphylatoxin levels in cord and adult blood. Furthermore we aimed to determine if the entire complement cascade could be activated by lactic acidosis. Cord and adult blood samples (n = 20 each) were collected and incubated for one hour in either untreated condition or with the addition of lactate in two concentrations (5.5 mmol/l vs. 22 mmol/l). Following incubation, levels of C3a, C5a and sC5b-9, and blood gas parameters were determined. Anaphylatoxin (C3a and C5a) and sC5b-9 levels increased with the addition of lactate in a dose-dependent manner in cord and adult blood (C3a: 1 h, 5.5 mmo/l, 22 mmol/l: 418/498/622 microg/l in cord blood; 1010/1056/1381 microg/l in adult blood, p<0,05; similar results were found for C5a and sC5b-9). Lactic acidosis leads to an activation of the entire complement system in neonates and in adults. This activation is dose-dependent and more pronounced in adults as compared to neonates.
References
Sep 1, 1992·European Journal of Pediatrics·L SchrodM Kirschfink
Sep 1, 1992·Journal of Pediatric Surgery·M HirthlerM Dickson
Mar 1, 1988·Circulation Research·R D RossenS Weakley
Sep 1, 1985·Immunobiology·S Zapf, M Loos
Dec 1, 1994·Shock·R H TurnageK T Oldham
Feb 1, 1996·American Journal of Surgery·J BakkerJ L Vincent
Jan 1, 1996·European Journal of Pediatrics·A EnskogL Larsson
Mar 1, 1996·Pediatric Research·F BrusS O Bambang
Feb 4, 1997·Circulation·G HorstickO Kempski
Jan 1, 1997·Archives of Disease in Childhood. Fetal and Neonatal Edition·S A Deshpande, M P Platt
Mar 1, 1997·Pediatric Research·E P ZilowG Zilow
May 13, 1998·Archives of Disease in Childhood. Fetal and Neonatal Edition·J SonntagM Obladen
Jul 15, 1998·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·I S MorganP S Mankad
Dec 5, 1998·Mediators of Inflammation·J SonntagM Obladen
Feb 2, 1999·Mediators of Inflammation·M EmeisM Obladen
May 24, 2000·Intensive Care Medicine·M HatherillI A Murdoch
Citations
Mar 6, 2007·Indian Journal of Pediatrics·Anna Petrova, Rajeev Mehta
Mar 17, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Janis R BedarfJuliane K Unger
Jun 1, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Rita S JerathGregory G Passmore
Jun 29, 2015·Neuroscience·D AilC Grimm
May 23, 2015·Frontiers in Immunology·Hany Ibrahim KenawyAlan Bevington
Sep 23, 2003·Shock·Janos SzebeniPaul D Mongan