PMID: 9999Sep 28, 1976

In vitro activation of glycoprotein hormones. Hybridization of subunits from thyrotropin, lutropin and human choriogonadotropin

Biochimica Et Biophysica Acta
J C. PernolletR Salesse


In vitro assembly of thyrotropin alpha and beta subunits led to an increase in content of alpha helix and beta sheet very similar to that found for gonadotropins. This association-dependent active folding involved the burying of three tyrosine residues tentatively assigned to Tyr alpha 41, Tyr beta 37 and Tyr beta 59 and common to all studied glycoprotein hormones. In vitro hybridizations between alpha and beta subunits of various hormones (thyrotropin, lutropin and choriogonadotropin) from different species (ovine, bovine and human) triggered the same molecular events as assembly of homologous subunits: the burying of three tyrosine residues and the increase of periodic structure of the folding. These changes are slow, time-dependent processes. Rates and yields of hybrid formation measured by sedimentation analysis and difference spectroscopy of tyrosines are identical, within experimental error, with the rates and yields measured by the recovery of the biological activity either the stimulation of chick thyroids for thyrotropin-beta hybrids or binding to porcine testis receptors for gonadotropin-beta hybrids. Whatever the origin of the alpha subunit, the thyrotropin-beta hybrids were not able to bind to testis receptors altho...Continue Reading


Feb 1, 1975·Biological Reviews of the Cambridge Philosophical Society·M Wallis
Sep 1, 1974·FEBS Letters·P De la LlosaM P De la Llosa-Hermier
Aug 1, 1974·Archives of Biochemistry and Biophysics·K C InghamH Edelhoch
May 26, 1972·Biochemical and Biophysical Research Communications·L T Hunt, M O Dayhoff
May 21, 1974·Biochemistry·V G HumK F Mori
Aug 1, 1968·Archives of Biochemistry and Biophysics·B ShomeJ G Pierce
Aug 1, 1966·Biochemistry·A Tachibana, T Murachi


Feb 1, 1980·Biological Reviews of the Cambridge Philosophical Society·G Csaba
Mar 15, 2015·Frontiers in Endocrinology·Claire CahoreauY Combarnous
Sep 27, 1979·Nature·J MarchelidonY A Fontaine

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