PMID: 7544755Sep 1, 1995Paper

In vitro activation of woodchuck lymphocytes measured by radiopurine incorporation and interleukin-2 production: implications for modeling immunity and therapy in hepatitis B virus infection

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
P J Cote, J L Gerin

Abstract

Cellular immune responses to hepatitis B virus (HBV) play an important role in the resolution of acute infection. They also influence the course of chronic infection and disease but are inadequate to completely clear the infection. Woodchuck hepatitis virus (WHV) infection of the woodchuck can provide a model to study these processes. Lymphocyte responses of woodchucks were assessed by in vitro proliferation and/or interleukin (IL)-2 assays using mitogen (Concanavalin A [ConA]), cytokine (IL-2), superantigen (Staphylococcus aureus enterotoxin B [SEB]), major histocompatibility complex (MHC) allo-antigen (mixed lymphocyte reaction [MLR]), and viral antigens (woodchuck hepatitis virus core antigen [WHcAg] and woodchuck hepatitis virus surface antigen [WHsAg]). ConA-stimulated woodchuck lymphocytes underwent cell division based on cell counting experiments and produced IL-2 as detected using an IL-2-dependent murine cell line but failed to incorporate sufficient tritiated thymidine; however, they did incorporate sufficient tritiated adenosine and deoxyadenosine to permit development of a meaningful proliferation assay. The IL-2 assay was sensitive and specific for detection of woodchuck IL-2 induced by mitogen, superantigen, and M...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D R MilichA McLachlan
Aug 1, 1988·Journal of Hepatology·C FerrariF Fiaccadori
Apr 1, 1968·Experimental Cell Research·S J Adelstein, C P Lyman
Sep 4, 1980·Nature·G Dennert
Jun 1, 1964·Comparative Biochemistry and Physiology·S J ADELSTEINR C O'BRIEN

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Citations

Apr 9, 2001·Veterinary Immunology and Immunopathology·J MaschkeH Grosse-Wilde
Jun 8, 2002·Veterinary Immunology and Immunopathology·Stephan MenneBud C Tennant

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