In vitro activity and killing effect of the synthetic hybrid cecropin A-melittin peptide CA(1-7)M(2-9)NH(2) on methicillin-resistant nosocomial isolates of Staphylococcus aureus and interactions with clinically used antibiotics

Diagnostic Microbiology and Infectious Disease
Andrea GiacomettiGiorgio Scalise

Abstract

The in vitro activity of CA(1-7)M(2-9)NH(2), a 15-residue synthetic hybrid peptide derived from the sequences of cecropin A and melittin, alone and in combination with amoxicillin-clavulanate, imipenem, clarithromycin, ciprofloxacin, rifampin, and vancomycin, was investigated against 40 nosocomial isolates of methicillin-resistant Staphylococcus aureus. Antimicrobial activity of CA(1-7)M(2-9)NH(2) was measured by minimal inhibitory concentration, MBC, and time-kill studies. All isolates were inhibited at concentrations of 1 to 16 microg/mL. Combination studies performed with S. aureusATCC 43300 demonstrated synergy only when CA(1-7)M(2-9)NH(2) was combined with amoxicillin-clavulanate and imipenem. Our findings show that CA(1-7)M(2-9)NH(2) is active against methicillin-resistant S. aureusand that its activity is enhanced when it is combined with several antimicrobial agents.

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Citations

Oct 15, 2008·Antimicrobial Agents and Chemotherapy·Hiromi Sato, Jimmy B Feix
May 5, 2006·Applied and Environmental Microbiology·Rafael FerreEduard Bardají
Oct 22, 2011·International Journal of Molecular Sciences·Seong-Cheol ParkKyung-Soo Hahm
Mar 17, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Yu ZhangLinlin Hao
Jun 5, 2010·Expert Review of Anti-infective Therapy·Marco Cassone, Laszlo Otvos
Dec 10, 2015·Proteomics·Pramod ShahChien-Sheng Chen
Apr 4, 2021·Biomolecules·Christina WangMire Zloh
Mar 11, 2009·Biochimica Et Biophysica Acta·Evan F HaneyHans J Vogel

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