In vitro activity and mode of action of phenolic compounds on Leishmania donovani

PLoS Neglected Tropical Diseases
Christine Achiaa AntwiTheresa Manful Gwira

Abstract

Leishmaniasis is a disease caused by the protozoan parasite, Leishmania. The disease remains a global threat to public health requiring effective chemotherapy for control and treatment. In this study, the effect of some selected phenolic compounds on Leishmania donovani was investigated. The compounds were screened for their anti-leishmanial activities against promastigote and intracellular amastigote forms of Leishmania donovani. The dose dependent effect and cytotoxicity of the compounds were determined by the MTT assay. Flow cytometry was used to determine the effect of the compounds on the cell cycle. Parasite morphological analysis was done by microscopy and growth kinetic studies were conducted by culturing cells and counting at 24 hours intervals over 120 hours. The cellular levels of iron in promastigotes treated with compounds was determined by atomic absorption spectroscopy and the effect of compounds on the expression of iron dependent enzymes was investigated using RT-qPCR. The IC50 of the compounds ranged from 16.34 μM to 198 μM compared to amphotericin B and deferoxamine controls. Rosmarinic acid and apigenin were the most effective against the promastigote and the intracellular amastigote forms. Selectivity index...Continue Reading

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Citations

Apr 11, 2020·PLoS Neglected Tropical Diseases·Wandayi Emmanuel AmlabuTheresa Manful Gwira
Aug 18, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Nagham Naddaf, Shaden Haddad
Jan 26, 2021·Journal of Experimental Pharmacology·Hulubanchi NigatuAbel Degu
Feb 25, 2020·Free Radical Biology & Medicine·Andrew J GhioMichael C Madden
Mar 5, 2021·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Layane Valéria AmorimFernando Aécio de Amorim Carvalho
Mar 26, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Bruna Taciane da Silva BortoletiWander Rogério Pavanelli
Apr 4, 2021·Biochimica Et Biophysica Acta. General Subjects·Tiago H S SouzaAdriana Fontes
Apr 17, 2021·Journal of Nanobiotechnology·Humzah JamshaidGul Majid Khan
Jun 24, 2021·PLoS Pathogens·Carla Gilabert CarbajoCalvin Tiengwe
Jul 2, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jéssica Adriana JesusLuiz Felipe Domingues Passero
Jul 4, 2021·Biochimie·Simone S C OliveiraPatrícia Severino
Aug 7, 2021·Frontiers in Pharmacology·João Victor Silva-SilvaFernando Almeida-Souza

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Methods Mentioned

BETA
flow cytometry
atomic absorption spectroscopy
PCR
fluorescence microscopy

Software Mentioned

GraphPad Prism
ImageJ
Primer
FlowJo

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