In-vitro activity of azithromycin in against intracellular Helicobacter pylori

The Journal of Antimicrobial Chemotherapy
K HultenL Engstrand

Abstract

We studied the effect in vitro of azithromycin on the clinical strain Helicobacter pylori H:72 growing intracellularly in monolayers of HEp-2 epithelial cells. After using gentamicin to eradicate extracellular bacteria, different concentrations of azithromycin were added to the infected cells and samples were taken after 0, 4, 8 and 24 h. Infected cells not exposed to antibiotic were included as controls. The MIC of azithromycin to the H. pylori was 0.25 mg/L and the MBC 0.5 mg/L in a broth dilution plate count method. A bactericidal effect was observed on intracellular H. pylori, with inhibition increasing with increasing azithromycin concentrations. However, extracellular concentrations of 200 x MBC were necessary to achieve intracellular killing. Our results show that azithromycin is active against intracellular H. pylori suggesting that it might be possible to exploit this activity when treating infections due to the organism.

Citations

Jan 9, 1999·Alimentary Pharmacology & Therapeutics·W D CheyJ Scheiman
Mar 19, 2003·Journal of Clinical Gastroenterology·G K AnagnostopoulosD Arvanitidis
Apr 17, 2001·Antimicrobial Agents and Chemotherapy·R PiccolominiM Neri
Jun 23, 1999·Mayo Clinic Proceedings·S Alvarez-Elcoro, M J Enzler
Mar 29, 2007·Cellular Microbiology·Andre Dubois, Thomas Borén
May 10, 2003·FEMS Immunology and Medical Microbiology·Andreas Munk Petersen, Karen Angeliki Krogfelt
Jun 1, 2005·Pediatric Clinics of North America·Manuel R Amieva
Dec 1, 1996·Antimicrobial Agents and Chemotherapy·K HulténL Engstrand

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