In vitro activity of Caspofungin combined with Fluconazole on mixed Candida albicans and Candida glabrata biofilm

Medical Mycology
Siripen PeseeNissara Tanarerkchai

Abstract

The objective of this study was to evaluate the antifungal effect of caspofungin (CAS) combined with fluconazole (FLU) on the biofilm biomass and cultivable viability and microstructure of Candida albicans and Candida glabrata mixed biofilm in vitro. Biofilms were formed in a 96-well microtiter plate for crystal violet assay and colony forming unit (CFU) method and grown on plastic coverslip disks for scanning electron microscopy. MIC50 of CAS and FLU against single Candida spp. and mixed Candida spp. biofilms were evaluated using crystal violet assay. Additional, C. albicans and C. glabrata mixed biofilms were incubated with subinhibitory CAS concentration plus FLU and their percentages of Candida biofilm reduction were calculated. We found that percentages of biofilm reduction were significantly decreased when CAS at 0.25MIC and FLU (0.25 or 0.5MIC) were combined (P < .05) but not different when CAS at 0.5 MIC combined with FLU at 0.25 or 0.5MIC, compared to CAS treatment alone. Structural analyses revealed that CAS/FLU combination-treated biofilms showed less hyphae and blastospores with some aberrant cells compared to control group. Although it was evident that a greater CFU of Candida glabrata were demonstrated in every gr...Continue Reading

References

Sep 12, 2000·The Journal of Antimicrobial Chemotherapy·G S Baillie, L J Douglas
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Oct 18, 2002·Antimicrobial Agents and Chemotherapy·Stefano P BachmannJosé L López-Ribot
Jun 26, 2003·Trends in Microbiology·Frank C OddsNeil A R Gow
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Stefano P BachmannJosé L López-Ribot
Feb 26, 2004·Antimicrobial Agents and Chemotherapy·Melissa D JohnsonJohn H Rex
Apr 14, 2004·Journal of Applied Microbiology·M A El-AziziN Khardori
Jan 18, 2005·Clinical Microbiology Reviews·Pranab K MukherjeeMahmoud A Ghannoum
Nov 30, 2006·Antimicrobial Agents and Chemotherapy·Jeniel NettDavid Andes
Aug 8, 2007·Archives of Oral Biology·Zaw M TheinLakshman P Samaranayake
Dec 25, 2007·Journal of Microbiological Methods·Elke PeetersTom Coenye
Mar 13, 2009·Journal of Dental Research·J M ten CateP W J de Groot
May 5, 2009·Microbes and Infection·Fahmi HasanBettina C Fries
May 16, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David L HornKaren M Webster
Oct 30, 2009·Critical Reviews in Microbiology·Gordon RamageJose Lopez-Ribot
Dec 10, 2009·The Journal of Antimicrobial Chemotherapy·Selma TobudicElisabeth Presterl
May 26, 2010·The Journal of Infectious Diseases·Jeniel E NettDavid R Andes
Dec 17, 2010·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Sónia SilvaDavid W Williams
Feb 24, 2011·Antimicrobial Agents and Chemotherapy·Athanasios ChatzimoschouEmmanuel Roilides
May 5, 2011·Medical Mycology·Heather T TaffDavid R Andes
Mar 23, 2012·Journal of Applied Oral Science : Revista FOB·Apurva K PathakPallavi Shrivastva
Jan 1, 2013·Annals of Transplantation : Quarterly of the Polish Transplantation Society·Chun-Hua YangXiang-Dong Guan
Jan 16, 2013·Antimicrobial Agents and Chemotherapy·K F MitchellD R Andes
Nov 13, 2013·Antimicrobial Agents and Chemotherapy·Semanti SarkarJose L Lopez-Ribot

❮ Previous
Next ❯

Citations

Sep 2, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jean-Christophe LagierDidier Raoult
Feb 16, 2021·Critical Reviews in Microbiology·Hui LuYuanying Jiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.