In Vitro Activity of Omadacycline, a New Tetracycline Analog, and Comparators against Clostridioides difficile.

Antimicrobial Agents and Chemotherapy
Khurshida BegumKevin W Garey

Abstract

Omadacycline is a potent aminomethylcycline with in vitro activity against Gram-positive, Gram-negative, and anaerobic bacteria. Preliminary data demonstrated that omadacycline has in vitro activity against Clostridioides difficile; however, large-scale in vitro studies have not been done. The purpose of this study was to assess the in vitro susceptibility of omadacycline and comparators on a large biobank of clinical C. difficile isolates. In vitro C. difficile susceptibility to omadacycline and comparators (fidaxomicin, metronidazole, and vancomycin) was assessed using the broth microdilution method. Minimum bactericidal concentrations (MBCs) and time-kill assays were assessed for pharmacodynamics analysis, and whole-genome sequencing was performed in a subset of isolates to assess distribution of MICs and resistance determinants. Two hundred fifty clinical C. difficile isolates collected between 2015 and 2018 were tested for in vitro susceptibility of omadacycline and comparators. Ribotypes included F001 (n = 5), F002 (n = 56), F014-020 (n = 66), F017 (n = 8), F027 (n = 53), F106 (n = 45), and F255 (n = 17). Omadacycline demonstrated potent in vitro activity with an MIC range of 0.016 to 0.13 μg/ml, an MIC50 of 0.031 μg/ml, ...Continue Reading

References

Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Apr 16, 2005·Clinical Microbiology Reviews·Daniel E Voth, Jimmy D Ballard
Mar 20, 2008·Genome Research·Daniel R Zerbino, Ewan Birney
Sep 17, 2010·Nature·Sarah A KuehneNigel P Minton
Jul 20, 2011·Diagnostic Microbiology and Infectious Disease·Diane M Citron, Ellie J C Goldstein
May 9, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sarah B DoernbergHenry F Chambers
Sep 27, 2013·The New England Journal of Medicine·David W EyreA Sarah Walker
May 7, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stuart JohnsonUNKNOWN Polymer Alternative for CDI Treatment (PACT) investigators
Jan 30, 2015·Journal of Clinical Microbiology·Jonathan N V MartinsonSeth T Walk
Feb 13, 2015·PLoS Computational Biology·Xavier Didelot, Daniel J Wilson
Feb 26, 2015·The New England Journal of Medicine·Fernanda C LessaL Clifford McDonald
Feb 15, 2018·Antimicrobial Agents and Chemotherapy·Laure StapertChris Pillar
Mar 22, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L Clifford McDonaldMark H Wilcox
Feb 23, 2019·Open Forum Infectious Diseases·Bradley T EndresKevin W Garey
Aug 2, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James A KarlowskyGeorge G Zhanel
Aug 2, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Keith A Rodvold, Manjunath P Pai
Jan 14, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Konstantinos S KechagiasMatthew E Falagas
Feb 11, 2020·Emerging Microbes & Infections·Anne J Gonzales-LunaKevin W Garey
Feb 14, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael J SatlinJohn Turnidge

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Citations

Aug 25, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Keeley O'GradyThomas V Riley

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Methods Mentioned

BETA
PCR
ribotyping

Software Mentioned

Velvet
PhyML
BEST
ClonalFrameML
BLAST

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