In vitro activity of radezolid against Enterococcus faecium and compared with linezolid.

The Journal of Antibiotics
Zhichao XuJinxin Zheng

Abstract

This study aims to explore the antimicrobial activity and resistance mechanism of radezolid against Enterococcus faecium, and to compare it with linezolid. A total of 232 E. faecium isolates were collected, and the minimal inhibitory concentrations of radezolid and linezolid were determined. The radezolid- or linezolid-nonsusceptible isolates were selected by passage in vitro under antibiotic pressure. Oxazolidinone-resistant chromosomal genes and plasmid-borne genes cfr, optrA, and poxtA were detected by PCR and sequenced. Radezolid MIC90 was 4 times lower than linezolid in the 232 E. faecium isolates, including the linezolid-nonsusceptible isolates. This study found that 6.5% (15/232) of the E. faecium isolates carried the plasmid-borne genes cfr and 9.5% (22/232) carried the optrA gene, but only one of these isolates had a linezolid MIC ≥ 4 mg l-1. Among the 13 isolates with linezolid MIC ≥ 4 mg l-1 or radezolid MIC ≥ 1 mg l-1, genetic mutations in the V domain of 23S rRNA were only found in four isolates. The MICs of linezolid or radezolid against three E. faecium isolates increased to 4-16 times of the initial MICs after 140 days of daily passage in drug-containing medium. The radezolid MICs remained 8-16 times lower than ...Continue Reading

References

Dec 15, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jiyong YangYanping Luo
Oct 19, 2016·Current Opinion in Microbiology·Jeshina JanardhananShahriar Mobashery
Jun 1, 2015·Journal of Global Antimicrobial Resistance·Ingo KlareGuido Werner
May 16, 2017·Cell·François LebretonMichael S Gilmore
Nov 5, 2017·Journal of Global Antimicrobial Resistance·Ruru BiBing Gu
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Jun 8, 2018·Expert Opinion on Pharmacotherapy·Nicholas J MercuroErica S Herc
Sep 5, 2018·Infection·Maria J G T VehreschildGerd Fätkenheuer

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Citations

Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

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