PMID: 10602728Dec 22, 1999Paper

In vitro activity of riboflavin against the human malaria parasite Plasmodium falciparum

Antimicrobial Agents and Chemotherapy
T AkompongKasturi Haldar

Abstract

The human malaria parasite Plasmodium falciparum digests hemoglobin and polymerizes the released free heme into hemozoin. This activity occurs in an acidic organelle called the food vacuole and is essential for survival of the parasite in erythrocytes. Since acidic conditions are known to enhance the auto-oxidation of hemoglobin, we investigated whether hemoglobin ingested by the parasite was oxidized and whether the oxidation process could be a target for chemotherapy against malaria. We released parasites from their host cells and separately analyzed hemoglobin ingested by the parasites from that remaining in the erythrocytes. Isolated parasites contained elevated amounts (38.5% +/- 3.5%) of oxidized hemoglobin (methemoglobin) compared to levels (0.8% +/- 0.2%) found in normal, uninfected erythrocytes. Further, treatment of infected cells with the reducing agent riboflavin for 24 h decreased the parasite methemoglobin level by 55%. It also inhibited hemozoin production by 50% and decreased the average size of the food vacuole by 47%. Administration of riboflavin for 48 h resulted in a 65% decrease in food vacuole size and inhibited asexual parasite growth in cultures. High doses of riboflavin are used clinically to treat cong...Continue Reading

References

Feb 1, 1979·Experimental Parasitology·M J FriedmanW Trager
Aug 1, 1978·British Journal of Haematology·T MatsukiY Tani
Aug 20, 1976·Science·W Trager, J B Jensen
May 9, 1977·Biochemical and Biophysical Research Communications·T YubisuiY Yoneyama
Dec 1, 1991·Molecular and Biochemical Parasitology·S R MeshnickH Z Pan
Nov 1, 1991·The Journal of Clinical Investigation·P J RosenthalD Rasnick
May 1, 1990·The American Journal of Clinical Nutrition·B S DasT K Bose
Jan 1, 1990·Biochemical Pharmacology·K BeckerR H Schirmer
Nov 9, 1985·Lancet·P DuttaR Rivlin
Dec 7, 1985·Lancet·D I Thurnham
Feb 1, 1987·Molecular and Biochemical Parasitology·E HempelmannU Gröschel-Stewart
Nov 1, 1987·The American Journal of Tropical Medicine and Hygiene·W B CowdenF Yoneda
Nov 20, 1973·Biochemistry·A Mansouri, K H Winterhalter
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·D I ThurnhamR Bull
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·P Kaikai, D I Thurnham
Jan 1, 1994·Molecular and Biochemical Parasitology·Y L HongS R Meshnick
Oct 1, 1993·Seminars in Cell Biology·D E Goldberg
Nov 13, 1998·The Journal of Biological Chemistry·D J SullivanD E Goldberg

❮ Previous
Next ❯

Citations

Oct 2, 2012·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Kailash C Pandey
Dec 12, 2003·Molecular and Biochemical Parasitology·Tyler N BennettPaul D Roepe
Apr 29, 2004·Metabolic Engineering·Wilbert SybesmaJeroen Hugenholtz
Aug 21, 2013·Parasitology·Alice S ButterworthKatharine R Trenholme
Oct 7, 2004·Applied and Environmental Microbiology·Catherine BurgessDouwe van Sinderen
Apr 25, 2012·Journal of Tropical Medicine·Kailash C Pandey, Rajnikant Dixit
Jul 20, 2006·Microbial Cell Factories·Catherine M BurgessDouwe van Sinderen
Dec 5, 2012·International Journal of Molecular Sciences·Sandro PercárioMichael D Green
Feb 24, 2016·Journal of Agricultural and Food Chemistry·Kiran ThakurSachinandan De
Mar 10, 2009·European Journal of Medicinal Chemistry·Amit BhattacharyaVirendra K Bhasin
Dec 10, 2008·Acta Tropica·Lokesh C MishraVirendra K Bhasin
Mar 9, 2007·Journal of Inorganic Biochemistry·Susan M ChemalyRobyn L van Zyl
Feb 14, 2004·Redox Report : Communications in Free Radical Research·R Heiner SchirmerBocar Kouyaté
Dec 6, 2008·Biochimica Et Biophysica Acta·S HackettT G St Pierre
Sep 26, 2003·The Proceedings of the Nutrition Society·Nina L Etkin
May 8, 2020·Future Medicinal Chemistry·Ishan WadiAbhinav Sinha
Feb 7, 2020·International Journal of Molecular Sciences·Nittiya SuwannasomHans Bäumler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Related Papers

Antimicrobial Agents and Chemotherapy
Sanjay KumarUday Bandyopadhyay
European Journal of Biochemistry
Katja BeckerR H Schirmer
Transactions of the Royal Society of Tropical Medicine and Hygiene
D Morley
Archives of Biochemistry and Biophysics
S E PattisonR Beckley
© 2021 Meta ULC. All rights reserved