In vitro adaptation of Plasmodium falciparum reveal variations in cultivability

Malaria Journal
John WhiteP K Rathod

Abstract

Culture-adapted Plasmodium falciparum parasites can offer deeper understanding of geographic variations in drug resistance, pathogenesis and immune evasion. To help ground population-based calculations and inferences from culture-adapted parasites, the complete range of parasites from a study area must be well represented in any collection. To this end, standardized adaptation methods and determinants of successful in vitro adaption were sought. Venous blood was collected from 33 P. falciparum-infected individuals at Goa Medical College and Hospital (Bambolim, Goa, India). Culture variables such as whole blood versus washed blood, heat-inactivated plasma versus Albumax, and different starting haematocrit levels were tested on fresh blood samples from patients. In vitro adaptation was considered successful when two four-fold or greater increases in parasitaemia were observed within, at most, 33 days of attempted culture. Subsequently, parasites from the same patients, which were originally cryopreserved following blood draw, were retested for adaptability for 45 days using identical host red blood cells (RBCs) and culture media. At a new endemic area research site, ~65% of tested patient samples, with varied patient history and ...Continue Reading

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Citations

Jul 25, 2019·Antimicrobial Agents and Chemotherapy·Rimi ChakrabartiPradipsinh K Rathod
Dec 12, 2019·Malaria Journal·Gabriela ValenzuelaFabián E Sáenz
Dec 9, 2020·The American Journal of Tropical Medicine and Hygiene·Lidia Madeline MontenegroTatiana M Lopera-Mesa
Dec 22, 2017·Emerging Topics in Life Sciences·Catherine J Merrick

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Methods Mentioned

BETA
blood draw
light microscopy
blood collection
blood draws

Software Mentioned

REDCap
GraphPad Prism
LabKey

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