In vitro adhesion of Acanthamoeba castellanii to soft contact lenses depends on water content and disinfection procedure

Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association
Julia F RevereyChristine Selhuber-Unkel

Abstract

To compare the potential of different soft contact lenses to be contaminated with Acanthamoeba castellanii as a function of material parameters and cleaning procedures. Different unworn soft hydrogel and silicone hydrogel contact lenses were incubated with human pathogenic A. castellanii. The adhesion of the acanthamoebae was investigated on the contact lenses and put into relation to their material parameters. The efficacy of a recommended contact lens cleaning procedure in reducing A. castellanii adhesion was investigated. We found that material parameters such as elastic modulus, silicone content, ionic properties and swelling do not influence the adhesion of acanthamoebae to soft contact lenses. A material parameter that influenced adhesion significantly was the water content of the lens. With increasing water content, the adhesion of acanthamoebae increased. By following the cleaning instructions of the manufacturer the contamination of the lenses with A. castellanii could be reduced to a minimum, as shown both on contact lenses and in control experiments. With this study we show that for the tested lenses, the adhesion of A. castellanii to contact lenses is independent of the silicone content of the lens, but depends nonl...Continue Reading

References

Mar 1, 1990·The British Journal of Ophthalmology·D F LarkinD L Easty
Jan 1, 1995·Optometry and Vision Science : Official Publication of the American Academy of Optometry·D V SealA Tomlinson
May 1, 1996·Archives of Ophthalmology·A I GorlinD G Ahearn
Dec 22, 1999·Microbes and Infection·J Y NiederkornJ P McCulley
Jul 7, 2000·The Journal of Antimicrobial Chemotherapy·N A TurnerD Lloyd
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·R Hughes, S Kilvington
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Feb 10, 2009·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Simon Kilvington, James Lonnen
Jun 12, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Md Kamruzzaman PramanikShigehiko Yumura
Feb 26, 2013·Trends in Parasitology·Jacob Lorenzo-MoralesBasilio Valladares

❮ Previous
Next ❯

Citations

May 5, 2017·PloS One·Steven HuthChristine Selhuber-Unkel
Dec 7, 2017·Eye & Contact Lens·Berna B Bakay, Zubeyde A Polat
May 10, 2020·Pathogens·Edyta B HendigerJacob Lorenzo-Morales
Apr 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Amy XiaoRajamani Lakshminarayanan
Sep 24, 2014·Beilstein Journal of Nanotechnology·Sören B GutekunstChristine Selhuber-Unkel
Oct 30, 2020·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Ana Gomes de Lacerda, Madalena Lira
Mar 30, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Mark WillcoxLyndon Jones
Apr 16, 2019·ACS Biomaterials Science & Engineering·Sören B GutekunstChristine Selhuber-Unkel
Mar 28, 2020·ACS Biomaterials Science & Engineering·Michael TimmermannChristine Selhuber-Unkel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.