In vitro affinity maturation of HuCAL antibodies: complementarity determining region exchange and RapMAT technology

Immunotherapy
Josef PrasslerStefanie Urlinger

Abstract

Monoclonal antibodies gain ever-increasing importance in the treatment of human diseases across a broad range of indications. Diverse technologies currently exist, which are used to generate recombinant therapeutic antibodies that are basically indistinguishable from naturally occurring human immunoglobulins. We describe how human combinatorial antibody libraries are used together with unique optimization techniques to produce such therapeutically relevant proteins, for instance in the areas of oncology and inflammation.

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Citations

Sep 29, 2020·Frontiers in Immunology·Mohamed A AlfalehAnwar M Hashem
May 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dirk PonselKathrin Tissot

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Methods Mentioned

BETA
ELISA
fluorescence-activated cell sorter
phage-display
phage display
nuclear
x-ray crystallography
gel-filtration
surface plasmon resonance
PCR

Software Mentioned

RapMAT
Biacore

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