In vitro aging of boar spermatozoa: role of sperm proximity and seminal plasma

Andrology
A Luther, Dagmar Waberski

Abstract

Knowledge on the effect of seminal plasma on sperm function in extended semen during in vitro storage is lacking. The aim was to examine the interactive role of sperm concentration and seminal plasma concentration on boar sperm function during in vitro aging. Experiment 1: Twenty-one boar ejaculates were aliquoted with Beltsville Thawing Solution into five semen doses containing between 32.5 and 8.5 × 106  sperm/mL. Experiment 2: Semen samples (n = 8) containing 18 × 106 or 10 × 106  sperm/mL with their natural amount of seminal plasma and 10 × 106  sperm/mL substituted with autologous seminal plasma to the same concentration as in doses with 18 × 106  sperm/mL were prepared. Experiment 3: Four variants of semen doses containing 18 × 106 or 10 × 106  sperm/mL with either 10% or 0.5% (v/v) seminal plasma were used. Lipid peroxidation was assessed using Bodipy 581/591 in samples (n = 8) with two different sperm concentrations. Semen was examined during 144-h storage at 17 °C by computer-assisted semen analysis and flow cytometry. Experiment 1: 3D kinematic patterns revealed a concentration- and time-dependent loss of sperm kinematics in samples with < 23 × 106  sperm/mL (p < 0.05). Percent viable spermatozoa with high mitochondri...Continue Reading

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Citations

Sep 10, 2020·International Journal of Molecular Sciences·Lisa HöfnerDagmar Waberski
Feb 1, 2020·Animal Reproduction Science·Lisa HöfnerDagmar Waberski

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