In vitro amoebacidal activity of photodynamic therapy on Acanthamoeba

The British Journal of Ophthalmology
Z ChenL Ran

Abstract

To evaluate the effectiveness of Hypocrellins B (HB) photodynamic therapy (PDT) on Acanthamoeba trophozoites and cysts, and its cytotoxic potential on the corneal epithelial cells and stromal cells in vitro. Acanthamoeba trophozoites and cysts (5 x 10(4)/ml) were incubated with various concentrations of HB, followed by exposure to light (>470 nm) for 30 min at a fluence rate of 50 mW/cm(2). Survival rate was assessed using the live/dead viability/cytotoxicity assay and non-nutrient agar--Escherichia coli culture assay. Rabbit corneal epithelial cells and stromal cells were incubated with various concentrations of HB, followed by exposure to the same light fluence rate as above. The corneal cell survival rate was assessed using the live/dead viability/cytotoxicity assay. HB-PDT showed a dose-dependent inhibition on the trophozoites and cysts. The 100% inhibitory concentration (IC(100)) of HB was 1 microg/ml for the trophozoites and 20 microg/ml for the cysts. The 50% inhibitory concentration (IC(50)) of HB for the cysts was 3.8 microg/ml and for the trophozoites 0.23 microg/ml. The inhibition of the HB-PDT on the rabbit corneal cells and stromal cells was observed, but HB without irradiation had no inhibition on the corneal cell...Continue Reading

Citations

Feb 22, 2012·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·N SzentmáryB Seitz
Nov 28, 2012·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Renata T KashiwabuchiPeter J McDonnell
Sep 26, 2013·Current Opinion in Pharmacology·Rui YinMichael R Hamblin
Mar 11, 2015·Antimicrobial Agents and Chemotherapy·Yousuf AqeelNaveed Ahmed Khan
Oct 21, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Tobias HagerN Szentmáry
Dec 3, 2020·Translational Vision Science & Technology·Lei ShiNóra Szentmáry
Feb 23, 2021·Lasers in Surgery and Medicine·Yunialthy Dwia PertiwiTakemasa Sakaguchi

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