In Vitro Amoebicidal Activity of Titanium Dioxide/UV-A Combination Against Acanthamoeba

Investigative Ophthalmology & Visual Science
Gabrielle GomartA Sauer

Abstract

To assess the amoebicidal effect of titanium dioxide (TiO2)/UV-A combination against Acanthamoeba sp trophozoites and cysts. The amoebicidal effect of the TiO2/UV-A combination was tested on trophozoites and cysts of clinical isolates of Acanthamoeba hatchetti and Acanthamoeba sp genotype T4, obtained from two severe cases of ulcerative keratitis. Samples of cultured Acanthamoeba were transferred to a 96-well plate. We tested the effect of sterile water (blank control), TiO2 alone, UV-A alone, TiO2 and additional UV-A exposure, chlorhexidine 0.02% alone, chlorhexidine 0.02% and TiO2, chlorhexidine and UV-A, chlorhexidine 0.02% and TiO2, and additional UV-A exposure. Cell viability assessment was done using the trypan blue dye exclusion method. The combination of TiO2 with UV-A demonstrated antitrophozoite and anticyst activity (P < 0.05). This in vitro study showed a synergistic effect of the association of chlorhexidine with TiO2 and UV-A on cysts (P < 0.001). Given the in vitro synergistic effectiveness of the association of chlorhexidine with TiO2 and UV-A against cysts, the treatment of Acanthamoeba keratitis could be improved by this new therapeutic approach.

References

Jul 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·T SaitoT Morioka
Jun 1, 1969·The Journal of Cell Biology·B Bowers, E D Korn
Dec 1, 1995·The British Journal of Ophthalmology·C D IllingworthD L Easty
Jul 5, 2002·Clinical Microbiology Reviews·Frederick L Schuster
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Aug 6, 2004·Free Radical Research·Kazutaka HirakawaShosuke Kawanishi
Feb 3, 2005·Luminescence : the Journal of Biological and Chemical Luminescence·Xing-Zheng WuKeiko Akiyama
Feb 28, 2006·Trends in Parasitology·Daniel W Clarke, Jerry Y Niederkorn
Jun 16, 2006·FEMS Microbiology Reviews·Naveed Ahmed Khan
Aug 11, 2006·Current Opinion in Ophthalmology·Kristin M Hammersmith
Apr 13, 2007·FEMS Immunology and Medical Microbiology·Govinda S VisvesvaraFrederick L Schuster
Oct 16, 2007·Applied and Environmental Microbiology·Gaëtan Gogniat, Sam Dukan
Feb 8, 2008·Experimental Parasitology·Münevver SökmenAlper Aslan
Oct 31, 2008·Talanta·C DodeigneR Lejeune
May 5, 2009·Ophthalmology·Anna S KitzmannMichael D Wagoner
Apr 28, 2011·Applied Microbiology and Biotechnology·Howard A FosterAlex Steele
May 15, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ryuichi NakanoYoshinobu Kubota
Apr 23, 2013·Clinical & Experimental Ophthalmology·Zubeyde A PolatMustafa K Arici
Nov 23, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hilary Humphreys
Feb 25, 2015·The Cochrane Database of Systematic Reviews·Majed AlkharashiShameema Sikder
Oct 29, 2015·Cornea·Lamprini PapaioannouMiltiadis Papathanassiou
Dec 17, 2015·Antimicrobial Agents and Chemotherapy·Yousuf AqeelNaveed Ahmed Khan
Jan 17, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hinojal ZazoJosé M Lanao
Jan 19, 2016·The Ocular Surface·Youngsub EomHyo Myung Kim
Mar 19, 2016·Cornea·Nicholas J R Maycock, Rakesh Jayaswal
Sep 8, 2016·Antimicrobial Agents and Chemotherapy·Ruqaiyyah SiddiquiNaveed Ahmed Khan
May 10, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Nasser K AwadYosry S Morsi
Jul 14, 2017·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Hidemasa NakaminamiNorihisa Noguchi
Jan 11, 2018·Parasites & Vectors·Ayaz AnwarRuqaiyyah Siddiqui
Mar 28, 2018·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Shokufeh TavassoliKieren Darcy

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