PMID: 8604550Nov 1, 1995Paper

In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR

Veterinary Microbiology
Y R Chávez GonzálezK E Johansson

Abstract

Mycoplasma bovis and Mycoplasma agalactiae are two very closely related species which cause mastitis in cows and goats, respectively. M. bovis can also cause arthritis and respiratory disease in cattle. It has recently been shown that the 16S rRNA sequences differ only in 8 nucleotide positions between the two species [J.G. Mattsson, B. Guss and K.-E. Johansson (1994) FEMS Microbiol. Lett., 115: 325-328]. These nucleotide differences are distributed over the molecule in such a way that it is difficult to design specific identification systems, based on PCR only, for M. bovis and M. agalactiae. Two different PCR systems based on 16S rRNA sequence data have, however, been designed for these two species. The forward primers were identical in the two systems and complementary to a segment of the evolutionarily variable region V2. The reverse primers were complementary to the variable region V6, in which there are two nucleotide differences between M. bovis and M. agalactiae. The size of the PCR products, generated with these primers, was 360 bp. Cross-amplification was obtained with the two species in the heterologous PCR systems, but with approximately a 100-fold lower efficiency. Cross-amplification was not obtained with any othe...Continue Reading

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Citations

Apr 11, 2001·Veterinary Microbiology·S TolaG Leori
Feb 20, 2002·Veterinary Microbiology·Malin Heldtander KönigssonKarl-Erik Johansson
Oct 7, 2008·New Zealand Veterinary Journal·J FitzmauriceJ S O'Keefe
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Jul 19, 2015·Pathogens and Disease·Shrilakshmi HegdeRohini Chopra-Dewasthaly
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