In vitro analysis of gadolinium chloride abrogation of the systemic tolerance induced by portal venous administration of ultraviolet B-irradiated donor cells

Transplantation
S YuM W Flye

Abstract

Normally, a Buffalo (BUF) recipient (RT1b) rejects a heterotopically transplanted Lewis (LEW) heart (RT1l) drained into the portal vein (PV) within 14 days. However, the addition of PV administration of 25x10(6) ultraviolet B (UVB)-treated LEW spleen cells (SC) to BUF recipients 7 days before cardiac transplantation results in 70% long-term allograft survival. In this study, we used gadolinium chloride (GdCl3) (7 mg/kg/day) to selectively block the phagocytosis of recipient hepatic Kupffer cells before PV injection of UVB-treated donor SC to examine the mechanism of tolerance induction, as measured by in vitro analysis of mixed lymphocyte culture (MLC), T cell cytotoxicity, T helper cell precursors (pTH), and cytotoxic T cell precursors (pCTL) by limiting dilution analysis. A BUF recipient that received untreated or gamma-irradiated LEW SC intraportally reacted to in vitro stimulation by LEW alloantigen with increased MLC proliferation, T cell cytotoxicity, pTH and pCTL frequencies, and interleukin-2 production. In contrast, SC from BUF that received UVB-treated LEW SC were hyporesponsive on MLC stimulation by donor LEW alloantigen and exhibited markedly reduced cytotoxicity, pTH and pCTL frequency, and interleukin-2 production...Continue Reading

References

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Citations

Jul 22, 2005·Immunology and Cell Biology·Kosei MaemuraZhaoli Sun
Apr 9, 2005·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J A Diaz-Peromingo, A Gonzalez-Quintela
Mar 14, 2009·Autoimmunity Reviews·Fredrik WermelingTracy L McGaha
Mar 17, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Christoph TroppmannRichard V Perez
May 22, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Nico SchaeferAndreas Türler
Jun 19, 2004·Transplant Immunology·Jong Kwon ParkRichard V Perez
Nov 18, 2018·International Journal of Molecular Sciences·Andrés D KleinSilvana Zanlungo

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