In vitro analysis of oocyte cumulus complex pickup rate in the hamster Mesocricetus auratus

Molecular Reproduction and Development
S HuangP Talbot

Abstract

In mammals, the oocyte and its surrounding cumulus cells constitute on oocyte cumulus complex (OCC). During ovulation, OCCs are extruded into the peritoneal or bursal cavity, depending on the species, and are then rapidly picked up by the fimbria on the outer surface of the oviductal infundibulum and transported to the ampulla, where fertilization occurs. We developed a method to measure OCC pickup rates quantitatively in vitro, and we used this method to evaluate the effects of viscosity and temperature on pickup rates. Hamster infundibula are placed in a holding pipette in a chamber modified to study OCC pickup. Ciliary beat frequencies (CBF) can be measured in the same preparation. Pickup rates vary depending on the pathway on which the OCC travels over the surface of the infundibulum; however, rates for a given pathway are very consistent. The average pickup rate at room temperature calculated from three different pathways/infundibulum was 55.2 +/- 10.6 microns/sec. Both rates between infundibula from the same female and rates among infundibula from different females were in most cases similar. Preparations preincubated in vitro for 2.75 hr produced rates similar to nonpreincubated samples, while longer preincubation result...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J T NorwoodR G Anderson
Mar 12, 1976·Science·S A HalbertR J Blandau
Feb 1, 1991·Biology of Reproduction·S S SuarezR L Powell
Jan 1, 1990·Toxicology and Applied Pharmacology·A M Cummings, S D Perreault
Jun 1, 1989·Biology of Reproduction·S A HalbertS E Szal
Aug 1, 1988·Clinical Science·R Wilson
Nov 1, 1988·Medical & Biological Engineering & Computing·G A HollowayW I Lee
Mar 1, 1988·Journal of Reproduction and Fertility·G D Moore, H B Croxatto
Jun 1, 1995·Annals of Medicine·M O Pulkkinen
Jun 1, 1995·The Journal of Experimental Zoology·G Di CarlantonioP Talbot
Nov 1, 1995·Reproductive Toxicology·T MagersT Hoodbhoy

❮ Previous
Next ❯

Citations

Mar 19, 2003·Reproductive Toxicology·Karen RivelesP Talbot
Jan 8, 1999·Molecular Biology of the Cell·P TalbotM Knoll
May 14, 2004·Reproductive Biology and Endocrinology : RB&E·Karen RivelesPrue Talbot
Sep 30, 2005·Reproductive Biology and Endocrinology : RB&E·Prue Talbot, Karen Riveles
Jan 24, 2007·Traffic·Søren T ChristensenPeter Satir
Apr 3, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Prudence Talbot
Apr 29, 2005·Molecular Reproduction and Development·Stefan C TeilmannSøren T Christensen
Apr 10, 2007·Annals of the New York Academy of Sciences·I ZervomanolakisL Wildt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.