In vitro analysis of the terminator T(II) of the inhibitor antisense rna II gene from plasmid pMV158

Plasmid
Gloria del Solar, Manuel Espinosa

Abstract

One element involved in the copy number control of plasmid pMV158 is the antisense RNA II. We have determined the precise size of this RNA synthesized in vitro. Transcript termination occurs at two consecutive template positions within a typical rho-independent terminator, T(II). Using a series of plasmid derivatives in which the T(II) terminator has been partially or totally removed, we have analyzed the relationship between the predicted stability of the RNA hairpin encoded by T(II) and the efficiency of in vitro intrinsic termination. All the plasmids of the pMV158 family, constituted so far of 19 replicons, harbor putative antisense RNA-encoding genes which share in common the relative location with respect to the essential rep gene, the small size, and the presence of rho-independent transcription terminators.

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Citations

Apr 3, 2003·Journal of Bacteriology·Tatiana Venkova-CanovaJan Nesvera
Jul 7, 2012·PloS One·Mónica Aguado-UrdaJosé F Fernández-Garayzábal
Feb 7, 2013·Research in Microbiology·Manuel Espinosa
Aug 4, 2018·Wiley Interdisciplinary Reviews. RNA·Radoslaw Pluta, Manuel Espinosa
Sep 19, 2008·Biotechnology Letters·Eun Ju Yang, Hae Choon Chang
Jun 25, 2015·Microbiology Spectrum·J A Ruiz-MasóG Del Solar

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