In vitro and clinical data analysis of Osteopontin as a prognostic indicator in colorectal cancer

Journal of Cellular and Molecular Medicine
Ran WeiHang Fai Kwok

Abstract

Osteopontin (OPN) has been shown to promote colorectal cancer (CRC) progression; however, the mechanism of OPN-induced CRC progression is largely unknown. In this study, we found that OPN overexpression led to enhanced anchorage-independent growth, cell migration and invasion in KRAS gene mutant cells but to a lesser extent in KRAS wild-type cells. OPN overexpression also induced PI3K signalling, expression of Snail and Matrix metallopeptidase 9 (MMP9), and suppressed the expression of E-cadherin in KRAS mutant cells. In human CRC specimens, a high-level expression of OPN significantly predicted poorer survival in CRC patients and OPN expression was positively correlated with MMP9 expression, and negatively correlated with E-cadherin expression. Furthermore, we have found that 15 genes were co-upregulated in OPN highly expression CRC and a list of candidate drugs that may have potential to reverse the secreted phosphoprotein 1 (SPP1) gene signature by connectivity mapping. In summary, OPN is a potential prognostic indicator and therapeutic target for colon cancer.

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Citations

Aug 23, 2019·Cancer Biotherapy & Radiopharmaceuticals·Xiaofei XuMingqing Shi
Sep 11, 2019·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mourad AssidiJaudah Al-Maghrabi
Nov 15, 2020·Oral Diseases·Erison Santana Dos SantosAdriana Franco Paes Leme
Dec 17, 2020·Frontiers in Molecular Biosciences·Tengteng WeiDonglin Zhu
Mar 21, 2020·Cancer Research·Casimiro GerarduzziElliot Drobetsky
Nov 27, 2020·Frontiers in Molecular Biosciences·Changsheng YangLujun Shen
Jan 21, 2021·Cancers·Pia GamradtAna Hennino
Nov 19, 2020·Cancers·Hannah R MoormanKebin Liu
Jul 9, 2021·Journal of Bone Oncology·Ali NazarizadehMasoumeh Tavakoli-Yaraki

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Datasets Mentioned

BETA
GSE14333
GSE17538
GSE
14333

Methods Mentioned

BETA
Infection
Protein Assay

Software Mentioned

PubMed
sscMap
R scripting
Leica
ImageJ
R script
QUADrATiC system
Statistically Significant Connection ' s Map ( sscMap )

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