In vitro and in vivo activity of polyclonal and monoclonal human immunoglobulins G, M, and A against Pseudomonas aeruginosa lipopolysaccharide.

Infection and Immunity
G B PierH Zweerink

Abstract

We evaluated the in vitro opsonophagocytic killing activity of monoclonal human immunoglobulin G (IgG), IgM, and IgA specific for Pseudomonas aeruginosa lipopolysaccharide and the in vivo protective capacity in neutropenic mice of both monoclonal and purified polyclonal IgG, IgM, and IgA. Monoclonal IgM was efficacious in mediating opsonophagocytic killing only in conjunction with complement, whereas monoclonal IgG opsonic killing was potentiated by complement, and monoclonal IgA opsonic killing was independent of complement. These findings are similar to those previously reported for purified polyclonal IgM, IgG, and IgA. The monoclonal and polyclonal immunoglobulins had comparable 50% protective doses in neutropenic mice (range, 0.28 to 0.46 microgram per mouse). The protective activity of IgM in neutropenic mice was abolished by cobra venom factor treatment, whereas IgG and IgA maintained efficacy in cobra venom factor-treated mice. These data indicate that all three major human serum immunoglobulin isotypes have opsonophagocytic and protective activities against P. aeruginosa, with a critical role for complement in the function of IgM.

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Citations

Mar 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xuemei XieJ Christopher Hall
Mar 1, 2014·Expert Review of Vaccines·Gregory P Priebe, Joanna B Goldberg
Apr 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J Verhoef, R Torensma
Sep 24, 2011·Human Vaccines·Anurag SharmaStefan Worgall
Jun 1, 1993·FEMS Immunology and Medical Microbiology·A B LangS J Cryz
Dec 1, 1996·Trends in Microbiology·J B Goldberg, G B Pler
May 1, 1995·Infection and Immunity·C H ModyD E Woods

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