In vitro and in vivo acute response towards injectable thermosensitive chitosan/TEMPO-oxidized cellulose nanofiber hydrogel

Carbohydrate Polymers
Trang Ho Minh NguyenByong-Taek Lee

Abstract

TEMPO-oxidized cellulose nanofiber (TOCNF) is a natural material with many promising properties, including biocompatibility and degradability. In this study, we integrated TOCNF at different concentrations (0.2, 0.4, 0.6, 0.8% w/v) with chitosan (CS) and created a thermosensitive injectable hydrogel intended for biomedical applications. These hydrogels can undergo sol-gel transition at body temperature through interactions between chitosan and β-glycerophosphate. The addition of TOCNF resulted in faster gelation time and increased porosity. These hydrogels with TOCNF showed improved biocompatibility both in vitro and in vivo compared to CS hydrogel. Both MC3T3-E1 pre-osteoblast cells and L929 fibroblast cells showed biocompatibility towards CS/TOCNF 0.4. After 7days of implantation, initial inflammatory response to CS/TOCNF 0.4 was found. Such response was significantly subsided within 14days. Cell infiltration within the hydrogel was also prominent, showing anti-inflammatory or wound healing (M2) macrophage at 14days after implantation. These results showed that the addition of TOCNF could significantly improve the biocompatibility of CS hydrogel as a biomaterial for biomedical application.

Citations

May 16, 2019·Journal of Biomedical Materials Research. Part a·Pavel A MarkovSergey V Popov
Nov 5, 2019·Journal of Biomaterials Applications·Hai Van HoByong-Taek Lee
Jan 28, 2020·Current Medicinal Chemistry·Avik KhanYonghao Ni
Dec 1, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir SheikhiAli Khademhosseini
Jan 1, 2020·Carbohydrate Polymers·Shaohua ZhangJinmei He
Mar 9, 2021·Frontiers in Bioengineering and Biotechnology·Peipei FengSongze Wu
May 11, 2021·Macromolecular Bioscience·Zhuqing Li, Kaitlin M Bratlie

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