Abstract
The present study aimed to evaluate in vitro and in vivo antibacterial activity of hydroxyl radical generation system by photolysis of H(2)O(2), which is a new disinfection system for the treatment of oral infection diseases such as periodontitis developed in our laboratory. Firstly, generation of the hydroxyl radical by the photolysis of H(2)O(2) in which 1 mol l(-1) H(2)O(2) was irradiated with a dual wavelength-light emitting diode (LED) at wavelengths of 400 and 465 nm was confirmed by applying an electron spin resonance-spin trapping technique. Secondly, the bactericidal effect of the system was examined under a similar condition in which Staphylococcus aureus suspended in 1 mol l(-1) H(2)O(2) was irradiated with LED light, resulting in substantial reduction of the colony forming unit (CFU) of the bacteria within a short time as 2 min. Finally, in vivo antibacterial effect of the photolysis of H(2)O(2) on a rat model of S. aureus infection was evaluated by a culture study. Since a significant reduction of recovered CFU of S. aureus was obtained, it is expected that in vitro antibacterial effect attributable to hydroxyl radicals generated by photolysis of H(2)O(2) could be well reflected in in vivo superficial bacterial inf...Continue Reading
References
Jan 1, 1986·Annual Review of Physiology·W A Pryor
Oct 26, 2005·The Journal of Surgical Research·Muthusamy Senthil KumarPraveen Kumar Sehgal
Mar 15, 2006·The Journal of Antimicrobial Chemotherapy·Osnat FeuersteinDoron Steinberg
Jul 31, 2007·The Journal of Surgical Research·Muthusamy Senthil KumarPraveen Kumar Sehgal
Mar 18, 2009·Antimicrobial Agents and Chemotherapy·Yukiko Okuyama-NishidaShunji Natori
Sep 29, 2009·PloS One·Rosanna CapparelliDomenico Iannelli
Oct 2, 2009·Journal of Clinical Biochemistry and Nutrition·Atsuo IwasawaYoshimi Niwano
Oct 17, 2009·Journal of Ethnopharmacology·Joseph A O OlugbuyiroO O Leigh
Mar 18, 2010·The Journal of Antimicrobial Chemotherapy·Emily HartRoy Robins-Browne
Oct 6, 2010·Antimicrobial Agents and Chemotherapy·Hiroyo IkaiMasahiro Kohno
Jul 27, 2011·Infection and Immunity·Daniel PrantnerUma M Nagarajan
Jul 27, 2011·PloS One·Guy ZinmanPenelope A Morel
Oct 8, 2011·Journal of Clinical Biochemistry and Nutrition·Masahiro KohnoYoshimi Niwano
Oct 26, 2011·Antimicrobial Agents and Chemotherapy·Midori ShiratoYoshimi Niwano
Citations
Nov 28, 2013·PloS One·Hiroyo IkaiYoshimi Niwano
Oct 1, 2013·Biocontrol Science·Hiroyo IkaiYoshimi Niwano
Jun 26, 2013·Biocontrol Science·Akira OyamadaYoshimi Niwano
Sep 1, 2014·Applied Biochemistry and Biotechnology·Makoto TsuruyaKeiichi Sasaki
Oct 5, 2013·Journal of Chemotherapy·Yu OdashimaYoshimi Niwano
Oct 6, 2014·Journal of Bioscience and Bioengineering·Toshihide TokiYoshimi Niwano
Jun 28, 2013·FEMS Microbiology Reviews·Fatma VatanseverMichael R Hamblin
Oct 19, 2013·Biomatter·Erik UnossonKen Welch
Jul 7, 2015·PloS One·Hong ShengYoshimi Niwano
Sep 13, 2018·Nanoscale·Zhengqing YanXiaogang Qu
Sep 1, 2020·Photobiomodulation, Photomedicine, and Laser Surgery·Samir NammourKinga Grzech-Leśniak
Oct 31, 2015·Nanoscale·Hong MiaoXiaoming Yang
Sep 28, 2017·Scientific Reports·Taro KannoKeiichi Sasaki
Oct 7, 2017·Frontiers in Physiology·Yoshimi NiwanoMana Tsukada
Dec 8, 2020·Small·Yan ZhangYuanhong Xu
May 30, 2014·ACS Nano·Hanjun SunXiaogang Qu