In vitro and in vivo antibacterial activity of environmental bacteriophages against Pseudomonas aeruginosa strains from cystic fibrosis patients

Applied Microbiology and Biotechnology
Tomasz OlszakZuzanna Drulis-Kawa

Abstract

The goal of the study was to determine the relationship between in vitro/in vivo efficacy of environmental Pseudomonas phages and certain phenotypical properties of Pseudomonas aeruginosa (PA) strains. We studied the diversity between particular isolates and determined phage sensitivity in vitro and in vivo in the Galleria mellonella insect model. Twenty-eight lytic bacteriophages specific for PA were tested against 121 CF PA isolates including 29 mucoid PA strains. Most strains from cystic fibrosis (CF) patients were lysed by at least three phages (93.6 %), but completely insensitive strains were also present (6.4 %). Two phages PA5oct and KT28 exhibited high rates of lytic potency on 55-68 % of PA strains (72-86 % of mucoid isolates). We further explored phage activity against six PA strains (CF and non-CF) in vitro, comparing clonal differences in phage susceptibility with bacterial properties such as the ability to form biofilms, mucosity, twitching motility, and biochemical profiles. We observed the relationship between variation in phage susceptibility and Fourier transform infrared spectroscopy (FTIR) analysis in the spectra window of carbohydrates. The protective efficacy of two selected phages against PA PAO1 and 0038 ...Continue Reading

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Citations

Oct 23, 2016·Applied Microbiology and Biotechnology·Bartosz RoszniowskiZuzanna Drulis-Kawa
Jun 16, 2016·Scientific Reports·Katarzyna Danis-WlodarczykZuzanna Drulis-Kawa
Jun 27, 2017·Nature Reviews. Microbiology·Jeroen De SmetRob Lavigne
Nov 28, 2017·Scientific Reports·Tomasz OlszakZuzanna Drulis-Kawa
Oct 26, 2018·PloS One·Prasanth ManoharNachimuthu Ramesh
Feb 26, 2020·International Journal of Molecular Sciences·Mateusz SzymczakPiotr Golec
Mar 5, 2017·Postȩpy higieny i medycyny doświadczalnej·Fairoz Al-WrafyAndrzej Gamian
May 18, 2018·Frontiers in Microbiology·Giantommaso ScarasciaPei-Ying Hong
Nov 9, 2018·Frontiers in Cellular and Infection Microbiology·Lucy L FurfaroBarbara J Chang

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Methods Mentioned

BETA
infrared spectroscopy
electron microscopy
electrophoresis
phage collection
genotyping
transmission electron microscopy

Software Mentioned

R
Elmer
Perkin
GraphPad Prism
R Team

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