In vitro and in vivo anticarcinogenic effects of RNase MC2, a ribonuclease isolated from dietary bitter gourd, toward human liver cancer cells

The International Journal of Biochemistry & Cell Biology
Evandro Fei FangTzi Bun Ng

Abstract

Hepatocellular carcinoma (HCC) constitutes a predominant part of primary liver cancer which ranks as the fifth most common cancer as well as the third most common cause of cancer mortality. In view of the poor prognosis of unresectable liver cancers, it is of pivotal importance to develop novel chemotherapeutical regimens. RNase MC2 is a 14-kDa ribonuclease isolated from dietary bitter gourd (Momordica charantia) that manifested antitumor potential against breast cancers. In this study, we investigated the potential application of RNase MC2 on Hep G2 cells. We showed that RNase MC2 inhibited cell proliferation and induced cell apoptosis in both in vitro and in vivo studies. RNase MC2 treatment caused cell cycle arrest predominantly at the S-phase and apoptosis, which is associated with the activation of both caspase-8 and caspase-9 regulated caspase pathways. Our further investigation disclosed that RNase MC2 down-regulated the anti-apoptotic protein Bcl-2 and increased the expression of pro-apoptotic protein Bak. Moreover, the phosphorylation of ERK and JNK was involved in the apoptosis process. Importantly, RNase MC2 significantly suppressed the growth of Hep G2 xenograft-bearing nude mice by inducing apoptosis. This notion i...Continue Reading

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Citations

May 28, 2014·BioMed Research International·Takeo TatsutaKazuo Nitta
Oct 24, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Chia-Jung LiHsue-Yin Hsu
Jul 28, 2016·Seminars in Cancer Biology·Komal RainaRajesh Agarwal
Mar 13, 2016·Chinese Journal of Natural Medicines·Prasad R DandawateShrikant Anant
Nov 29, 2017·International Journal of Molecular Sciences·Shuo JiaJianhua Xie
Jun 23, 2018·Current Protein & Peptide Science·Evandro Fei FangTzi Bun Ng
May 30, 2019·Frontiers in Pharmacology·Massimo BortolottiLetizia Polito

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